| 鼻渊净胶囊的HPLC指纹图谱研究 |
| 投稿时间：2021-01-13 修订日期：2021-05-16 点此下载全文 |
| 引用本文：程艳芹,朱姗,李明春,张玉杰,付青姐.鼻渊净胶囊的HPLC指纹图谱研究[J].药学实践杂志,2021,39(5):431~436 |
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| 基金项目:军队医疗机构制剂标准提高科研专项课题重点项目（No.14ZJZ02） |
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| 中文摘要:目的 建立鼻渊净胶囊的高效液相色谱（HPLC）指纹图谱。方法 采用Agilent SB-C18（4.6 mm×250 mm，5 μm）色谱柱，乙腈-水为流动相、以1.0 ml/min流速行梯度洗脱，检测波长210 nm，柱温30 ℃，洗脱时间为80 min。采用中药色谱指纹图谱相似度评价系统（2004A版）对检测出色谱进行指纹图谱相似度评价。结果 建立了鼻渊净胶囊的HPLC指纹图谱，确定了20个共有峰，15个峰归属到各药材，其中5个峰确认了化学成分；10批样品的指纹图谱的整体相似度与对照图谱比较，均在90%以上。结论 所建立的鼻渊净胶囊指纹图谱有助于从整体上控制该制剂的质量。 |
| 中文关键词:鼻渊净胶囊 指纹图谱 连翘苷 蒙花苷 木兰脂素 欧前胡素 异欧前胡素 |
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| Study on HPLC fingerprints of Biyuanjing capsules |
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| Abstract:Objective To establish a HPLC fingerprints of Biyuanjing capsules.Methods The column was Agilent SB-C18(4.6mm×250 mm, 5 μm). The mobile phase was acetonitrile-water with gradient elution at a flow rate of 1.0 ml/min. The detection wavelength was 210 nm. The detection time was 80 min.Results The HPLC fingerprints of Biyuanjing capsules were established. Twenty common peaks were confirmed, of which, 15 peaks were belonging to each crude drug and 5 peaks were identified as chemical components. The overall similarity of the fingerprints of 10 batches of samples was above 90% comparing with the control.Conclusion This method can be used for the quality control of Biyuanjing capsules. |
| keywords:Biyuanjing capsules fingerprints phillyr in linarin magnolin imperatorin isoimperatorin |
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