| HPLC-ELSD法测定胃康颗粒中人参皂苷Rb1和黄芪甲苷的含量 |
| 投稿时间：2020-01-19 修订日期：2020-05-12 点此下载全文 |
| 引用本文：刘璐,于双雨,刘艳华.HPLC-ELSD法测定胃康颗粒中人参皂苷Rb1和黄芪甲苷的含量[J].药学实践杂志,2020,38(4):359~363 |
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| 基金项目:回医优势病种诊疗规范及回药特色产品开发研究（2017BY084） |
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| 中文摘要:目的 优化胃康颗粒中人参皂苷Rb1和黄芪甲苷的提取方法，并建立其含量测定方法。方法 以人参皂苷Rb1和黄芪甲苷的含量作为指标，采用单因素考察法对提取工艺进行优化；采用高效液相色谱-蒸发光散射法（HPLC-ELSD），XBridge®Shield RP18（4.6 mm×250 mm，5 μm）为色谱柱；乙腈-水（32：68）为流动相；柱温为30 ℃；漂移管温度为60 ℃，载气流量为1.7 SLM，建立测定胃康颗粒中人参皂苷Rb1和黄芪甲苷含量的方法。结果 当采用甲醇回流提取1.5 h，正丁醇提取5次，氨水洗涤2次时，人参皂苷Rb1和黄芪甲苷的提取含量较高；建立的HPLC-ELSD法测定人参皂苷Rb1和黄芪甲苷含量，线性关系良好（r > 0.9997），日内日间精密度均小于1%，加样回收率分别为95.65%和100.57%，稳定性和重复性的RSD均小于3%，含量分别为2.8630 mg/g和0.2576 mg/g，RSD分别为0.62%和1.51%。结论 优化了胃康颗粒中人参皂苷Rb1和黄芪甲苷的提取方法，建立了可靠、准确、重现性好的测定胃康颗粒中人参皂苷Rb1和黄芪甲苷含量的HPLC-ELSD方法。 |
| 中文关键词:胃康颗粒 黄芪甲苷 人参皂苷Rb1 含量测定 高效液相色谱-蒸发光散射法 |
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| Detectione of the contents of ginsenoside Rb1 and astragaloside IV in Weikang granules by HPLC-ELSD |
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| Abstract:Objective To optimize the extraction method and develop the detection method of ginsenoside Rb1 and astragaloside Ⅳ in Weikang granules.Methods The extraction process of ginsenoside Rb1 and astragaloside Ⅳ in Weikang granules were optimized by single factor investigation, with the contents of ginsenoside Rb1 and astragaloside Ⅳ as optimization indicators. The HPLC-ELSD method was developed for the detection of ginsenoside Rb1 and astragaloside Ⅳ in Weikang granules. Separation was carried out on an XBridge®Shield RP18 column (4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-water（32:68）at the flow rate of 1 ml/min. The column temperature was maintained at 30 ℃. The drift tube temperature was set at 60 ℃, and the carrier gas flow rate was 1.7 SLM.Results The optimized extraction methods of ginsenoside Rb1 and astragaloside Ⅳ in Weikang granules were as the following: methanol reflux extraction for 1.5 h, and n-butanol extraction and ammonia washed for 5 and 2 times, respectively. The HPLC-ELSD method was established to detect the contents of ginsenoside Rb1 and astragaloside Ⅳ. The linear relationship was good (r > 0.9997). The intra-day and inter-day precision was less than 1%. The recovery rates were 95.65% and 100.57%. The stability and repeatability RSD were less than 3%. The contents were 2.8630 mg/g and 0.2576 mg/g. The RSDs were 0.62% and 1.51%, respectively.Conclusion The extraction method of ginsenoside Rb1 and astragaloside Ⅳ in Weikang granules is optimized, and a reliable, accurate and reproducible HPLC-ELSD method for the detection of the contents of ginsenoside Rb1 and astragaloside Ⅳ in Weikang granules is established. |
| keywords:Weikang granules astragaloside Ⅳ ginsenoside Rb1 content determination HPLC-ELSD |
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