| 用HPLC法测定丹参素棕榈醇酯脂质体药物含量 |
| 投稿时间：2019-02-14 修订日期：2019-05-06 点此下载全文 |
| 引用本文：王晶,张川,栗意,任金妹,唐扣明.用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J].药学实践杂志,2019,37(5):450~452,480 |
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| 基金项目:上海市卫生和计划生育委员会科研项目资助（20164Y0101） |
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| 中文摘要:目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。方法 采用Agilent ZORBAX Eclipse Plus C18柱（4.6 mm×200 mm，5 μm）；以乙腈-水（50∶50）含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相，流速为1 ml/min，检测波长为280 nm，柱温为室温。结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求，在0.05~0.45 mg/ml范围内线性关系良好（r=0.999 5，n=5），回收率在96%~102%之间，日内及日间RSD均小于2%（n=5）。3批样品的含量分别为97.81%、101.20%、98.53%。结论 该方法准确可靠、简单快速，可用于丹参素棕榈醇酯脂质体含量的测定。 |
| 中文关键词:丹参素 脂质体 高效液相色谱 包封率 |
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| Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method |
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| Abstract:Objective To establish a HPLC method for the assay of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (PDSS) in liposomes.Methods The separation was performed with an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm×200 mm,5 μm) at room temperature.The mobile phase was acetonitrile-aqueous solution (500 ml water + 0.1 ml triethylamine + 0.1 ml formic acid,pH=6-7) (50:50) with the flow rate of 1 ml/min.Zidovudine palmitate was detected at 280 nm.Results PDSS had a good linear recovery between 0.05-0.45 mg/ml.The intra-day and inter-day RSD were <2%.The extracted recovery was in the range of 96% to 102%.The assay results for three batches were 97.81%,101.20% and 98.53%. Conclusion This method is simple,accurate and reliable.It can be used for the determination of PDSS in liposomes. |
| keywords:Danshensu liposome HPLC entrapment efficiency |
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