| HPLC法测定皮肤病血毒片中7种成分的含量 |
| 投稿时间：2016-09-05 修订日期：2016-12-27 点此下载全文 |
| 引用本文：史志辉,肖会敏,何悦,刘洋,刘海月,杨旭,王四旺.HPLC法测定皮肤病血毒片中7种成分的含量[J].药学实践杂志,2017,35(5):427~432 |
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| 中文摘要:目的 建立同时测定皮肤病血毒片中7种成分含量的HPLC方法。方法 采用Kromasil C18 柱（250 mm×4.6 mm，5 μm）；流动相：乙腈-0.5%磷酸水溶液，梯度洗脱法；流速：0.8 ml/min；PDA检测器，检测波长：245、325、403 nm。结果 皮肤病血毒片中绿原酸、羟基红花色素A、芍药苷、连翘酯苷A、阿魏酸、小檗碱和甘草酸分别在10.00~200.00，4.00~80.00，20.00~400.00，4.00~80.00，3.00~60.00，10.00~200.00和10.00~200.00 μg/ml范围内呈良好的线性关系；各成分平均回收率均在95%~105%之间，RSD<2%。10批制剂中绿原酸、羟基红花色素A、芍药苷、连翘酯苷A、阿魏酸、小檗碱和甘草酸的平均含量（mg/片）与RSD（%）分别为0.299 5，2.25；0.700 0，1.33；0.429 2，1.21；0.039 1，2.34；0.014 8，2.23；0.209 0，2.06和0.272 7，2.68。结论 该方法准确可靠、简便快速，适用于皮肤病血毒片的质量控制。 |
| 中文关键词:皮肤病血毒片 高效液相色谱 绿原酸 羟基红花色素A 芍药苷 连翘酯苷A 小檗碱 甘草酸 |
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| Determination of seven components in Pifubingxuedu tablet by HPLC method |
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| Abstract:Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet. Methods The determination was performed on Kromasil-C18 column (4.6 mm×250 mm, 5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with a flow rate of 0.8ml/min. The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively. Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC. Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25%);0.700 0 mg/tablet(1.33%);0.429 2 mg/tablet (1.21%);0.039 1 mg/tablet (2.34%);0.014 8 mg/tablet(2.23%);0.209 0 mg/tablet (2.06%);0.272 7 mg/tablet (2.68%), respectively. Conclusion The established method is simple, convenient, accurate and reliable. It is suitable for the quality control of Pifubingxuedu tablet. |
| keywords:Pifubingxuedu tablet HPLC chlorogenic acid hydroxy Safflower Pigment A paeoniflorin forsythiaside A berberine glycyrrhizin acid |
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