| 反相高效液相色谱法测定人肝微粒体中帕洛诺司琼的含量 |
| 投稿时间：2005-01-11 点此下载全文 |
| 引用本文：陈长水,杨先启.反相高效液相色谱法测定人肝微粒体中帕洛诺司琼的含量[J].药学实践杂志,2005,(3):169~171 |
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| 中文摘要:目的 为了研究帕洛诺司琼的体外代谢,建立人肝微粒体中帕洛诺司琼的反相高效液相色谱测定法.方法 帕洛诺司琼与人肝微粒体共孵育之后,以Nova-park C18(5μm,200×4.6mm)柱为分析柱,甲醇-0.01 mol/L磷酸二氢钾缓冲盐(80:20,v/v)为流动相,流速1.0mL/min,紫外检测波长为240nm.结果 帕洛诺司琼在5～100μmol/L范围内线性关系良好(r=0.999 8).检测限为0.2μmol/L(S/N≥),定量限为1.0μmol/L(RSD=4.81%,n=3).方法回收率为96.0%～10.0%,日内、日间RSD分别<7.0%和<10%(n=5).结论 此法简便,准确,可用于研究帕洛诺司琼的体外代谢. |
| 中文关键词:帕洛诺司琼 反相高效液相色谱法 药物代谢 人肝微粒体 |
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| Determination of palonosetron metabolism in human hepatic microsome by RP HPLC |
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| Abstract:Objective To establish a RP-HPLC method for determinate palonosetron in human hepatic microsomes.Methods Pal onosetron in human hepatic microsomal incubates was assayed on a Nova-park C18(5μm, 200×4.6mm) column with a mobile phase of methanol-0.01mol/L KH2PO4 (80:20, v/v) at a flow-rate of 1.0 mL/min.A UV-VIS detector was operated at 240nm.Results The assay was linear from 5-100 μmol/L for palonosetron (r= 0.999 8 ).The limit of detection was 0.2 μmol/L ( signal to noise ratio≥3) and the limit of quantification was 1.0μmol/L (RSD =4.88%,n=3).The method afforded recoveries of 96.0%-103.0% (n=5), and inter-day and intra-day variation coefficient were <7% and 10% (n=5).Conclusion The method is simple, accurate and can be used to study the metabolism of palonosetron in human hepatic microsomes. |
| keywords:palonosetron RP-HPLC metabolism microsomes |
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