| HPLC法测定柿蒂中金丝桃苷与齐墩果酸的含量 |
| 投稿时间：2004-09-01 点此下载全文 |
| 引用本文：郑一敏,胥秀英,杨艳红,傅善权.HPLC法测定柿蒂中金丝桃苷与齐墩果酸的含量[J].药学实践杂志,2005,(3):163~165 |
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| 中文摘要:目的 用高效液相色谱法测定柿蒂中金丝桃苷与齐墩果酸的含量.方法 金丝桃苷的含量采用Shimadzu C18柱(150×4.6mm,5μm),流动相为甲醇-0.2%H3PO4溶液(45:55),流速1.0mL/min,检测波长350nm,用外标法定量;采用Shimadzu C18柱(150×4.6mm,5μm),流动相为甲醇-0.2%H3PO4溶液(90:10),流速0.8 mL/min,检测波长220nm,用外标法定量,测定柿蒂中齐墩果酸的含量.结果 金丝桃苷的线性范围0.50-2.50μg,r=0.9998,回收率99.65%,RSD 1.24%;齐墩果酸的线性范围1.56-5.20μg,r=0.9990,回收率97.02%,RSD2.84%.结论 该方法简便,快速,线性关系良好,可作为柿蒂的含量测定. |
| 中文关键词:柿蒂 金丝桃苷 齐墩果酸 高效液相色谱法 |
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| Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC |
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| Abstract:Objective To determine the content of hyperoside and oleanolic acid in Diospyros kaki.Methods The HPLC method was used to determine the content of hyperoside and oleanolic acid in Diospyros kaki.The analysis of hyperoside was carried out on Shi madzu C18column (150mm×4.6mm,5μm).The mobile phase was MeOH-0.2%H3PO4(45:55).The flow-rate was 1.0mL/min.The detection wave-length was 350nm.The analysis of oleanolic acid was carried out on ShimadzuC18column(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3PO4(90:10).The flow -rate was 0.8 mL/min.The detection wave -length was 220nm.Results The liner range of hyperoside was 0.50-2.50μg and gave a correlation (r) of 0.9999.The recovery was 99.65% and RSD was 1.24 % .The liner range of oleanolic acid was1.56-5.20μg and gave a correlation (r) of 0.9990.The recovery was 97.02% and RSD was 2.84 % .Conclusion The method is simple, fast and had a good linear relationship.It is to determine the content of hyperoside and oleanolic acid in Diospyros kaki. |
| keywords:Diospyros kaki hyperoside oleanolic acid HPLC |
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