| HPLC对人血浆中葛根素及葛根黄酮的线性考察 |
| 投稿时间：2002-02-15 点此下载全文 |
| 引用本文：郭建平,李国栋,赵彤英,范伟.HPLC对人血浆中葛根素及葛根黄酮的线性考察[J].药学实践杂志,2003,(4):219~221 |
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| 中文摘要:目的 对葛根素及葛根黄酮进行人血浆的线性考察,据此检测葛根素、葛根黄酮及其制剂人体内的药动学行为。方法 用高效液相色谱法考察,色谱条件:流速=1mL·min-1;柱温:20℃;检测波长:250nm;进样量50μL;流动相:甲醇:水=25:75。结果 葛根素R=6.56,精密度试验RSD≤5.02%,平均回收率=(100.9±0.5)%,葛根素及葛根黄酮血浆测定的线性范围0.0295～0.708μg·mL-1。结论 用高效液相色谱法可作为葛根素、葛根黄酮及制剂人体内药动学的检测手段,为检测葛根黄酮及制剂人体内的药动学行为提供了方法。 |
| 中文关键词:高效液相色谱 葛根素 葛根黄酮 |
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| Linear determination of puerarin and isoflavones in Radix Puerariae in Plasma |
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| Abstract:OBJECTIVE To study the pharmacokinetics behavior of puerarin, isoflavones and its Products in human body, make the determination of puerarin and isoflavones in Radix Puerariae. METHODS Carry out the determination by HPLC. The chromatographic condition: the flow rate was 1mL·min-1. The temperature of the column was 20C:the detection wavelength was at 250nm. The mobile phase was consisted of methanol-water(25:75). RESULTS R = 6.56 RSD <5.02%. The average recoveries:(100.9 + 0.5)%, Linear range is 0.0295～0.708μg·mL-1 for puerarin and isoflavones in human plasma. CONCLUSIONS Pharmacokinetics of Puerarin and isoflavones in human plasma could be determined by HPLC, supplying the method for examining isoflavones in Radix Puerariae in human body. |
| keywords:HPLC puerarin isoflavones |
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