| 淫羊藿中黄酮苷类化合物的群体感应抑制作用研究 |
| 投稿时间：2024-09-29 修订日期：2025-01-14 点此下载全文 |
| 引用本文：江冼芮,段雅倩,刘畅,张成中.淫羊藿中黄酮苷类化合物的群体感应抑制作用研究[J].药学实践杂志,2025,43(4):169~173 |
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| 基金项目:国家自然科学基金青年科学基金项目（82104326） |
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| 中文摘要:目的 从淫羊藿中发现具有群体感应抑制作用的黄酮苷类成分，并对其活性进行评价。方法 首先测定淫羊藿中5个主要的黄酮苷类化合物（宝藿苷、淫羊藿苷、朝藿定A/B/C）及淫羊藿提取物的最小抑菌浓度（MIC）值，随后利用酶标仪测定对紫色杆菌CV026的紫色色素产生的抑制作用，再利用对铜绿假单胞菌 PAO1的生物被膜以及几丁质酶进行定量测定。结果 淫羊藿提取物及其主要成分具有群体感应抑制活性作用，其中，淫羊藿苷、朝藿定C（100 μg /ml）作用明显。结论 中药淫羊藿具有对紫色杆菌CV026和铜绿假单胞菌 PAO1的群体感应系统有抑制作用，其中，淫羊藿苷和朝藿定C有望发展成为一种群体感应抑制剂的新药。 |
| 中文关键词:淫羊藿 群体感应 紫色杆菌CV026 铜绿假单胞菌 PAO1 |
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| Quorum-sensing inhibition of flavonoid glycosides from Epimedium brevicornum |
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| Abstract:Objective To identify flavonoid glycosides with quorum sensing inhibitory activity from Epimedium brevicornum and evaluate their bioactivity. Methods The minimum inhibitory concentrations （MICs） of five major flavonoid glycosides （baohuoside, icariin, epimedin A/B/C） and the extract of E. brevicornum were firstly determined. Subsequently, the inhibitory effects on the production of purple pigments in Chromobacterium violaceum CV026 were measured. Additionally, the biofilm formation and chitin quantification of Pseudomonas aeruginosa PAO1 were assessed. Results The extract of E. brevicornum and its primary components exhibited significant quorum sensing inhibitory activity. Particularly, icariin and epimedin C demonstrated superior inhibitory activity. Conclusion E. brevicornum demonstrates the ability to inhibit the quorum sensing system of Chromobacterium violaceum CV026 and Pseudomonas aeruginosa PAO1. Furthermore, icariin and epimedin C （100 μg/ml） show promise for development into novel drugs for quorum sensing inhibitor. |
| keywords:Epimedium brevicornum Quorum sensing Chromobacterium violaceum CV026 Pseudomonas aeruginosa PAO1 |
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