| 高血压对大鼠心血管纤维化及sFRP2表达的影响 |
| 投稿时间：2024-09-25 修订日期：2025-01-13 点此下载全文 |
| 引用本文：徐尧,马春辉,李志勇.高血压对大鼠心血管纤维化及sFRP2表达的影响[J].药学实践杂志,2025,43(4):180~184 |
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| 基金项目:上海市科技创新行动计划（201409004600）；上海市自然科学基金（23ZR1477400） |
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| 中文摘要:目的 探讨自发性高血压对大鼠心脏和主动脉组织重构的影响，特别关注心脏和主动脉组织中胶原纤维、弹力纤维含量以及心脏中分泌卷曲蛋白2（sFRP2）的含量变化。方法 选取28周龄的SHR（Spontaneously Hypertensive rat）大鼠和同龄的WKY（Wistar-Kyoto rat）大鼠作为实验动物。通过计算心重指数来评估心脏重塑大体指标。采用热碱法从大鼠胸主动脉中分离胶原纤维和弹力纤维，并使用生化分析方法测定其含量。此外，通过H&E染色、天狼猩红染色和地衣红染色对左心室和胸主动脉的组织切片进行病理学评估。蛋白免疫印迹实验用于测定心脏组织中sFRP2蛋白的表达水平。结果 相较于WKY大鼠，SHR大鼠心重指数明显增加（P<0.001），生化分析和病理切片染色结果显示，SHR组主动脉中胶原纤维含量较WKY组高，而弹力纤维含量较低，但两组间的差异未达到统计学意义；SHR组心脏中胶原纤维含量较WKY组明显增高（P<0.01），蛋白免疫印迹实验结果显示，两组大鼠心脏组织中sFRP2蛋白的表达水平无显著差异。结论 在自发性高血压大鼠模型中，心脏及主动脉组织的重构现象可能涉及多种复杂的分子机制，而不仅仅是胶原纤维和弹力纤维含量的变化。自发性高血压病的进展及其导致靶器官损害的机制仍需进一步研究。 |
| 中文关键词:SHR大鼠 高血压 胶原纤维 弹力纤维 分泌卷曲蛋白2 |
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| Effect of hypertension on cardiovascular fibrosis and sFRP2 expression in rats |
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| Abstract:Objective To investigate the effect of spontaneous hypertension on the remodeling of cardiac and aortic tissues in rats, with special attention to the changes in the content of collagen fibers, elastic fibers and secreted Frizzled-related protein 2 （sFRP2） in cardiac and aortic tissues. Methods 28-week-old SHR rats （Spontaneously Hypertensive rats） and WKY （Wistar-Kyoto rats） of the same age were selected as experimental animals. Cardiac load was assessed by calculating the cardiac weight index. Collagen fibers and elastic fibers were isolated from the rat thoracic aorta by hot alkali method, and their content was determined by biochemical analysis. In addition, pathological evaluation of tissue sections of the left ventricle and thoracic aorta were performed by H&E staining, Sirius red staining, and lichen red staining. Western blotting was used to determine the expression level of sFRP2 protein in cardiac tissues. Results Compared with WKY rats, the heart weight index of SHR rats increased significantly （P<0.001）, and the results of biochemical analysis and staining of pathological sections showed that the content of collagen fibers in the aorta in the SHR group was higher than that in the WKY group, while the content of elastic fibers was lower, but the difference did not have statistical significance. The content of collagen fibers in the heart of the SHR group was significantly higher than that in the WKY group （P<0.01）. Western blotting showed that there was no significant difference in the expression level of sFRP2 protein in heart tissues between the two groups. Conclusion The remodeling of cardiac and aortic tissues in a rat model of spontaneous hypertension may involve complex molecular mechanisms, not just changes in the content of collagen fibers and elastic fibers. The detailed mechanism of the progression of spontaneous hypertension and target organs damage still need further investigation. |
| keywords:SHR rats hypertension collagen fibers elastic fibers sFRP2 |
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