| 小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究 |
| 投稿时间：2024-09-23 修订日期：2024-11-23 点此下载全文 |
| 引用本文：宋泽成,马闪闪,胡巧灵,仲华,王彦.小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究[J].药学实践杂志,2025,43(2):87~91 |
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| 基金项目:生物安全项目（145AHQ082021000X）；国家自然科学基金（82204470） |
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| 中文摘要:目的 研究小檗碱（BBR）与氟康唑（FLC）联合用药的体外抗FLC耐受白念珠菌作用。方法 利用微量液基稀释法测定FLC单用对8株白念珠菌的最低抑菌浓度（MIC）以确定其对FLC的敏感性；通过琼脂平皿纸片扩散实验从FLC敏感菌株中筛选出FLC耐受菌株；利用琼脂平皿纸片扩散实验考察BBR与FLC联合用药对FLC耐受白念珠菌的作用。结果 选取的8株白念珠菌皆为FLC敏感菌株，其MIC50值均<0.5 μg/ml；菌株Y0109、9821、7879、7654、9296在恒温培养48 h后抑菌圈内出现菌落生长，表现出对FLC的耐受现象；菌株Y0109与9821在BBR与FLC联用时，恒温培养48 h后抑菌圈内的菌落数随BBR浓度的升高而逐渐减少，抑菌圈随BBR浓度的升高而逐渐清晰，随FLC载药量的增大而增大，显示出剂量依赖关系。结论 BBR与FLC联合用药具有良好的抗FLC耐受白念珠菌效果。 |
| 中文关键词:白念珠菌 小檗碱 氟康唑 联合用药 药物耐受 |
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| Study on the effect of berberine combined with fluconazole on fluconazole-tolerant Candida albcians strains |
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| Abstract:Objective To investigate the combined effect of berberine （BBR） and fluconazole （FLC） on FLC-tolerant Candida albicans in vitro. Methods The sensitivity of 8 strains of Candida albicans to FLC was assessed by determining their minimal inhibitory concentration （MIC） using broth microdilution method. FLC-tolerant strains were screened from FLC-sensitive strains by disk diffusion assay. The effect of BBR combined with FLC on FLC-tolerant Candida albicans was investigated by disk diffusion assay. Results All eight strains of Candida albicans exhibited sensitivity to FLC, with minimal inhibitory concentration （MIC50） values below 0.5 μg/ml. Strains Y0109, 9821, 7879, 7654, and 9296 displayed colony growth in the inhibition zone after 48 h of constant temperature incubation, indicating FLC tolerance. When strains Y0109 and 9821 were subjected to a combination of BBR and FLC, the number of colonies within the inhibition zone decreased progressively with the increase of BBR concentration following a 48 h constant temperature culture. The inhibition zone became clear with the increasing of BBR concentration and increased with the increase of FLC loading, which showed a dose-dependent relationship. Conclusion The BBR combined with FLC demonstrated efficacy against FLC-tolerant strains. |
| keywords:Candida albicans berberine fluconazole drug combination drug tolerance |
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