| 血小板特异性Metrnl基因敲除小鼠模型的构建与验证 |
| 投稿时间：2024-09-11 修订日期：2024-12-26 点此下载全文 |
| 引用本文：陈灿昕,缪竹威,缪朝玉.血小板特异性Metrnl基因敲除小鼠模型的构建与验证[J].药学实践杂志,2025,43(3):117~123 |
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| 基金项目:国家自然科学基金重点项目（82030110） |
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| 中文摘要:目的 构建血小板特异性Metrnl基因敲除的小鼠（Plt-Metrnl-/-小鼠）模型。方法 基于Cre-LoxP系统利用Pf4-Cre小鼠与实验室前期构建的Metrnlloxp/loxp小鼠进行交配繁殖，得到目的Plt-Metrnl-/-小鼠。对该目的小鼠进行基因型鉴定，收集其血液及心、肝、脾、肺、肾、脑、结肠、骨髓组织，利用实时荧光定量PCR技术、蛋白免疫印迹实验，考察血小板特异性Metrnl敲除小鼠的敲除情况。结果 Plt-Metrnl-/-小鼠的血小板Metrnl蛋白水平显著低于对照组WT小鼠，其他外周血细胞及各组织mRNA水平、蛋白水平、血常规指标、生长发育一般情况与对照组WT小鼠无明显差异。结论 血小板特异性Metrnl敲除小鼠（Plt-Metrnl-/-小鼠）模型构建成功。 |
| 中文关键词:Metrnl 基因敲除小鼠 血小板 |
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| Construction and validation of a platelet-specific Metrnl gene knockout mouse model |
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| Abstract:Objective To construct the platelet-specific Metrnl gene knockout （Plt-Metrnl-/-）mice model. Methods Based on the Cre-LoxP system, Metrnlloxp/loxp mice, previously constructed in our laboratory, were mated with Pf4-Cre mice to generate Plt-Metrnl-/- mice. The genotypes of the offspring were identified, and tissues of the platelet, other peripheral blood cells, heart, liver, spleen, lung, kidney, brain, colon, and bone marrow were collected. The expression of the Metrnl gene in Plt-Metrnl-/- mice was investigated by quantitative real-time PCR and western blot. Also, the blood routine index was tested in Plt-Metrnl-/- mice. Results Compared with wild-type mice, the level of Metrnl protein in platelets was significantly decreased in Plt-Metrnl-/- mice. There was no significant difference in mRNA and protein levels of other peripheral blood cells and tissues, as well as in blood routine index, growth, and development between Plt-Metrnl-/- mice and WT mice. Conclusion Platelet-specific Metrnl knockout mice（Plt-Metrnl-/- mice）model was successfully constructed. |
| keywords:Metrnl gene-knockout mouse platelet |
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