| 丹参FBXL 基因家族的鉴定和表达模式分析 |
| 投稿时间：2024-07-15 修订日期：2024-10-24 点此下载全文 |
| 引用本文：姚瑞阳,于海征,李耀盺,张磊.丹参FBXL 基因家族的鉴定和表达模式分析[J].药学实践杂志,2024,42(11):461~470 |
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| 基金项目:国家自然科学基金（82304665）；国家资助博士后研究院人员计划（GZC20233561） |
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| 中文摘要:目的 基于基因组数据鉴定丹参F-box-LRR （FBXL ）基因家族，并对其进行生物信息学与表达模式分析，为进一步深入阐明其基因功能提供依据。方法 从丹参基因组数据库中鉴定出SmFBXL基因，运用生物信息学方法及在线工具分析其基因结构特征，启动子顺式作用元件，编码的蛋白理化性质、系统进化、组织表达等。结果 从丹参的基因组中共鉴定出104个SmFBXL基因（SmFBXL1~SmFBXL104），不均等分布于8条染色体上，上游启动子含有与植物抗逆、生长发育和激素应答等相关的顺式作用元件。构建丹参、拟南芥和大豆的FBXL 家族成员的系统发育树，将104个SmFBXL基因分为7个亚族。通过同源进化分析，猜测SmFBXL36可能参与防御病原菌入侵，SmFBXL86、SmFBXL79可能在调控丹参侧根生长中起重要作用，SmFBXL11、SmFBXL40可能调节下胚轴生长。转录组数据显示SmFBXL基因在丹参不同组织中差异表达，其中13个SmFBXL基因在根和叶中的表达水平较高，可作为后续研究SmFBXL基因家族的候选基因。结论 研究结果为进一步解析SmFBXL基因在丹参逆境响应及次生代谢产物生物合成中的调控机制提供了参考。 |
| 中文关键词:丹参 F-box-LRR基因家族 生物信息学 功能分析 |
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| Identification and expression pattern analysis of FBXL gene family in Salvia miltiorrhiza |
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| Abstract:Objective To identify and analyze the bioinformatics and expression patterns of the F-box-LRR（FBXL） gene family of Salvia miltiorrhiza based on genomic data, and provide a foundation for further elucidating its gene functions. Methods The SmFBXL gene was identified from the Salvia miltiorrhiza genomic database. Its gene structure features, promoter cis-acting elements, physicochemical properties of encoded proteins, evolutionary relationships, and tissue expression were analyzed by bioinformatics methods and online tools. Results A total of 104 SmFBXL genes were identified from the Salvia miltiorrhiza genome, unevenly distributed on 8 chromosomes, with upstream promoters containing cis-acting elements related to plant stress resistance, growth and development, and hormone response. A phylogenetic tree of the FBXL family members of Salvia miltiorrhiza, Arabidopsis thaliana, and Glycine max was constructed, dividing the 104 SmFBXL genes into 7 subfamilies. Through homologous evolution analysis, it was speculated that SmFBXL36 might be involved in defense against pathogen invasion, SmFBXL86 and SmFBXL79 might play important roles in regulating lateral root growth in Salvia miltiorrhiza, and SmFBXL11 and SmFBXL40 might regulate hypocotyl growth. Transcriptome data showed differential expression of SmFBXL genes in different tissues of Salvia miltiorrhiza, with 13 SmFBXL genes showing higher expression levels in roots and leaves, serving as candidate genes for further research on the SmFBXL gene family. Conclusion The research results provided a reference for further elucidating the regulatory mechanisms of SmFBXL genes in stress response and secondary metabolite biosynthesis in Salvia miltiorrhiza. |
| keywords:Salvia miltiorrhiza F-box-LRR gene family Bioinformatics Functional analysis |
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