| 侧流免疫层析定量检测方法的研究进展 |
| 投稿时间：2023-07-18 修订日期：2024-05-22 点此下载全文 |
| 引用本文：顾佳钰,胡馨儿,王晓飞,张颖,张海,曹岩.侧流免疫层析定量检测方法的研究进展[J].药学实践杂志,2024,42(7):273~277,284 |
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| 基金项目:国家自然科学基金资助项目（82174092）；上海市科委资助项目（21ZR1483000）；上海市浦江计划资助项目（21PJD083） |
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| 中文摘要:侧流免疫层析技术是一项新型的即时检测技术，采用层析膜和标记材料进行检测。该检测技术具有方便快捷、成本低廉的特点，因此被广泛应用于生物医药、疾病检测、食品安全、环境保护等多个领域。传统的侧流免疫层析需要使用肉眼观测，因此只能提供至多半定量的结果，并且有可能存在观测误差。随着各种类型的标记材料和灵敏检测仪器的出现及广泛应用，侧流免疫层析技术逐步实现了对待检测物的定量检测。通过综述识别元件、标记材料、检测仪器等，阐述侧流免疫层析检测系统的研究进展以及目前的应用情况。 |
| 中文关键词:侧流免疫层析|检测仪|生物标记 |
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| Research progress on quantitative detection methods of lateral flow immunochromatography assay |
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| Abstract:Lateral flow immunochromatography assay is a new instantaneous detection technology that employs a chromatographic membrane and labeling materials for detection. This detection technology is convenient, fast, and inexpensive, and is therefore widely used in a number of different fields, such as biomedicine, disease detection, food safety, environmental protection, and so on. Traditional lateral flow immunochromatography assay relied on visual observation and provided only qualitative or semi-quantitative results. By utilizing various types of markers and sensitive detection devices, lateral flow immunochromatography assay enables quantitative and multi-component detection of the analytes. The research progress on the lateral flow immunoassay detection system and its current applications in the context of recognition elements, labeling materials, and detection instruments were reviewed in this paper. |
| keywords:lateral flow immunochromatography assay|detector|biomarker |
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