| 负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架治疗创伤性脑损伤的研究 |
| 投稿时间：2024-06-19 修订日期：2024-09-25 点此下载全文 |
| 引用本文：迟文雅,袁艳,李伟林,吴茼妤,俞媛.负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架治疗创伤性脑损伤的研究[J].药学实践杂志,2025,43(2):67~74 |
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| 基金项目:国家自然科学基金（82273487）；全军医学科技青年培育计划（21QNPY051）；军民融合孵化专项（2022-RH17） |
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| 中文摘要:目的 制备温敏水凝胶支架负载骨髓间充质干细胞（BMSCs）及白藜芦醇脂质体（RSV-LIP）形成治疗单元，考察其对创伤性脑损伤的治疗效率。方法 提取大鼠BMSCs，制备RSV-LIP并进行表征，构建细胞模型考察BMSCs联合RSV-LIP的体外药效。将BMSCs和RSV-LIP装载于水凝胶，构建TBI小鼠模型并评价水凝胶对模型的治疗作用。结果 RSV-LIP粒径127.8 nm，电位-4.9 mV，包封率78.50%，载药量2.37%。细胞Live/Dead染色结果表明水凝胶具有良好的生物相容性。BMSCs联合RSV-LIP对TNF-α具有明显抑制作用，可以显著降低ROS水平，划痕实验中具有显著的促细胞迁徙作用。水凝胶与对照组相比，mNSS评分显著降低（P<0.01）、悬挂得分显著升高（P<0.001）、踏空率显著降低（P<0.001）。结论 BMSCs和RSV-LIP联合应用具有抗氧化应激、抗炎和促进神经干细胞迁徙的作用，将其负载于水凝胶支架后局部植入，可以改善TBI小鼠的运动感觉功能。 |
| 中文关键词:创伤性脑损伤 水凝胶 白藜芦醇脂质体 骨髓间充质干细胞 |
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| Hydrogel scaffolds loaded with bone marrow mesenchymal stem cells/resveratrol liposomes for traumatic brain injury treatment |
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| Abstract:Objective To prepare a thermosensitive hydrogel scaffold loaded with bone marrow mesenchymal stem cells（BMSCs） and resveratrol liposomes （RSV-LIP） to form a therapeutic unit and evaluate its treatment efficacy for traumatic brain injury （TBI）. Methods BMSCs were extracted from rats, and RSV-LIP was prepared and characterized. Cell models were constructed to investigate the pharmacological effects of BMSCs combined with RSV-LIP. BMSCs and RSV-LIP were then loaded into the hydrogel, and a TBI mouse model was established to evaluate the therapeutic effects of the hydrogel. Results The RSV-LIP had a particle size of 127.8 nm, a Zeta potential of -4.9 mV, an encapsulation efficiency of 78.50%, and a drug loading content of 2.37%. Live-dead staining indicated good biocompatibility of the hydrogel. The combination of BMSCs and RSV-LIP significantly inhibited TNF-α and reduced ROS levels, promoting cell migration in scratch assays. Compared to the control group, the hydrogel group showed significantly lower mNSS scores （P<0.01）, higher hanging scores （P<0.001）, and reduced stepping errors （P<0.001）. Conclusion The combination of BMSCs and RSV-LIP exhibited antioxidative stress, anti-inflammatory, and neurogenic cell migration-promoting effects. When loaded into a hydrogel scaffold and locally implanted, it could improve the motor and sensory functions in TBI mice. |
| keywords:traumatic brain injury hydrogels resveratrol liposomes bone marrow mesenchymal stem cells |
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