| 消风止痒颗粒通过降低白三烯水平对小鼠特应性皮炎急性瘙痒的治疗作用研究 |
| 投稿时间：2023-06-19 修订日期：2023-10-26 点此下载全文 |
| 引用本文：丁华敏,郭羽晨,秦春霞,宋志兵,孙莉莉.消风止痒颗粒通过降低白三烯水平对小鼠特应性皮炎急性瘙痒的治疗作用研究[J].药学实践杂志,2024,42(5):211~216 |
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| 基金项目:上海市浦东新区卫生系统优秀青年医学人才培养计划资助（PWRq2020-25） |
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| 中文摘要:目的 建立小鼠特应性皮炎急性瘙痒模型，研究消风止痒颗粒的止痒作用及其机制探讨。方法 采用诱导法制备特应性皮炎小鼠模型。小鼠每天先后用卡泊三醇和卵清蛋白（OVA）涂抹右耳致敏10 d，然后在小鼠右颊皮内注射OVA进行激发，致使小鼠出现急性瘙痒，记录小鼠激发后30~60 min的抓挠次数。实验分为5组：空白对照组、模型组、消风止痒颗粒低剂量组（7.2 g/kg）和高剂量组（14.4 g/kg）、阳性对照组（孟鲁司特5 mg/kg）。各组于激发前12 h及30 min各灌胃给药一次。通过Elisa检测小鼠血清中白三烯水平，通过流式细胞术检测血液中嗜碱性粒细胞比例及激活状态。结果 小鼠经致敏和激发后出现明显的抓挠行为，模型组小鼠激发后30~60 min内抓挠次数均值为56次，消风止痒颗粒低和高剂量组小鼠抓挠次数均值分别为42次和23次，较模型组均显著降低（P<0.05）。消风止痒颗粒低、高剂量组血清中白三烯水平和嗜碱性粒细胞比例与模型组比较，均明显降低（P<0.05）。结论 消风止痒颗粒对小鼠特应性皮炎急性瘙痒具有一定治疗作用，作用机制与其降低特应性皮炎小鼠血清中白三烯水平和降低嗜碱性粒细胞比例有关。 |
| 中文关键词:消风止痒颗粒 特应性皮炎 瘙痒 嗜碱性粒细胞 白三烯 |
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| Therapeutic effect of Xiaofeng Zhiyang granules on acute itching in mice with atopic dermatitis by decreasing leukotriene |
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| Abstract:Objective To establish a mice model of atopic dermatitis with acute itching and investigate the antipruritic effect and its mechanism of Xiaofeng Zhiyang granules（XFZYG）. Methods A mice model of atopic dermatitis was prepared by induction method. Mice were sensitized by calcipotriol and ovalbumin (OVA) applying to the right ear daily for 10 days, and then stimulated by OVA injected intradermally into the right cheek to resulting in acute itching. These mice were divided into 5 groups: blank control group, model group, low dose (7.2 g/kg) and high dose (14.4 g/kg) of XFZYG, and positive control group (montelukast 5 mg/kg). Drugs were administered by gavage at 12 h and 30 min before stimulation. The leukotriene levels in the serum of the mice were measured by Elisa and the basophil ratio and activation status in the blood were measured by flow cytometry. Results The mean number of scratches in the model group was 56 between 30 min and 60 min after stimulation, while the mean number of scratches in the low and high dose of XFZYG groups were 42 and 23 respectively, which were significantly lower than those in the model group （P<0.05）. The serum leukotriene levels and the proportion of basophils in the low and high dose of XFZYG groups were significantly lower than those in the model group (P<0.05). Conclusion XFZYG had certain therapeutic effect on acute itching of atopic dermatitis in mice, and the mechanism of its action was related to the reduction of leukotriene level and basophil ratio in serum of mice with atopic dermatitis . |
| keywords:Xiaofeng Zhiyang granule atopic dermatitis pruritus basophil leukotriene |
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