| 中药青蒿抗氧化活性的谱效关系研究 |
| 投稿时间：2022-11-07 修订日期：2024-01-26 点此下载全文 |
| 引用本文：赖立勇,夏天爽,徐圣焱,蒋益萍,岳小强,辛海量.中药青蒿抗氧化活性的谱效关系研究[J].药学实践杂志,2024,42(5):203~210,216 |
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| 基金项目:国家自然科学基金（82174079，U1603283） |
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| 中文摘要:目的 通过考察11批青蒿的高效液相色谱（HPLC）指纹图谱与体外抗氧化活性的谱效关系，筛选出青蒿体外抗氧化作用的药效物质基础成分。方法 色谱柱为Aglient C18（250 mm×4.6 mm, 5 μm）色谱柱，流动相为0.2%磷酸水-甲醇，流速为1 ml /min；柱温为室温；检测波长为220 nm；进样量为10 μl。以异绿原酸A为参照，采用《中药指纹图谱相似度评价系统》（2012版）确定并记录11批青蒿样品的共有峰。检测不同青蒿样品对DPPH自由基和ABTS自由基的清除效率，作为其抗氧化评价，利用SIMCA14.1软件构建PLSR模型并分析谱效关系。结果 11批青蒿样品中共检测出48个共有峰，鉴定11个成分，分别为东莨菪内酯、滨蒿内酯、异绿原酸B、异绿原酸A、异绿原酸C、木犀草素、芹菜素、猫眼草黄素、青蒿素、艾黄素和青蒿酸。检测11批青蒿样品的DPPH自由基和ABTS自由基的清除能力，谱效关系显示，异绿原酸A、B、C和滨蒿内酯峰面积与青蒿的DPPH自由基和ABTS自由基清除能力正相关，且变量投影值大于1，表明这些成分在青蒿的体外抗氧化方面有显著贡献。结论 本研究考察了青蒿中不同物质的体外抗氧化能力，证明异绿原酸A、B、C和滨蒿内酯为青蒿的体外抗氧化活性的药效物质基础。 |
| 中文关键词:青蒿 高效液相色谱法 抗氧化 谱效关系 药效物质基础 |
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| Study on spectrum-effect relationship based on antioxidant activity of Artemisiae Annuae Herba |
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| Abstract:Objective To screen the pharmacodynamic material basic components of Artemisiae Annuae Herba and study its antioxidant activity in vitro by investigating the spectrum-effect relationship between the HPLC fingerprints of 11 batches of Artemisiae Annuae Herba (dried aerial part of Artemisia annua L.). Methods The determination was performed on Aglient C18 column (250 mm×4.6 mm, 5 μm) with mobile phase consisted of 0.2% phosphoric acid solution-Methanol (gradient elution) at the flow rate of 1.0 ml/min. The column temperature was indoor temperature, and detection wavelength was 220 nm, with sample size of 10 μl. Using isochlorogenic acid A as reference, HPLC fingerprints of 11 batches of samples were determined. The common peaks of 11 batches of samples were identified and recorded through TCM chromatographic fingerprint similarity evaluation system （2012 edition）. Using scavenging rate of DPPH and ABTS free radical as pharmacodynamic indicators of antioxidant effects, SIMCA 14.1 analysis software was used for PLSR to establish the spectra-effect relationship. Results There were 48 common peaks on 11 batches of sample, 11 components were identified as scopoletin, scoparone, isochlorogenic acid B, A, C, luteolin, apigenin, chrysosplenetin, artemisinin, artemisetin and artemisinic acid. The scavenging activity of 11 batches of samples to DPPH and ABTS free radicals was detected. The spectrum-effect relationship showed that isochlorogenic acid A, B, C and scoparone were positively associated with its antioxidant capacity, and variable projection value was greater than 1. It was suggested that these components were the material basis of antioxidant effect in Artemisiae Annuae Herba. Conclusion This study investigates the antioxidant capacity of different substances in Artemisiae Annuae Herba in vitro, and proves that isochlorogenic acid A,B，C and scoparone play a major role for the antioxidant capacity. |
| keywords:Artemisiae Annuae Herba HPLC antioxidant spectrum-effect relationship pharmacodynamic material basis |
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