| 全身过表达人METRNL基因小鼠模型的构建与验证 |
| 投稿时间：2023-11-08 修订日期：2024-02-17 点此下载全文 |
| 引用本文：王雪莲,郑斯莉,李志勇,罗亨宇,缪朝玉.全身过表达人METRNL基因小鼠模型的构建与验证[J].药学实践杂志,2024,42(5):198~202,222 |
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| 基金项目:国家自然科学基金重点项目（82030110，82330117）；国家自然科学基金青年科学基金项目（82104165）；上海市青年科技英才扬帆计划（21YF1457600） |
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| 中文摘要:目的 构建全身过表达人METRNL基因的小鼠模型（R26-L-METRNL+/-小鼠）。方法 基于Cre-loxP系统利用Dppa3-Cre小鼠和实验室前期构建的人METRNL基因条件性过表达（R26-LSL-METRNL+/-）小鼠进行交配繁殖，得到目标R26-L-METRNL+/-小鼠。将该目标小鼠进行基因型鉴定，收集其血液及心、肝、脾、肺、肾、脑、白色脂肪和肌肉组织，利用实时荧光定量PCR技术、蛋白免疫印迹实验和血清酶联免疫吸附实验，考察人METRNL基因在小鼠的表达情况。结果 R26-L-METRNL+/- 小鼠的人METRNL在组织mRNA水平、组织蛋白水平和血液蛋白浓度方面都有显著表达，远高于野生对照组小鼠。结论 R26-L-METRNL+/-小鼠模型构建成功。 |
| 中文关键词:分泌蛋白 全身过表达 小鼠 |
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| Construction and validation of a mouse model with systemic overexpression of human METRNL gene |
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| Abstract:Objective To generate mice with whole-body overexpression of human METRNL gene. Methods Based on Cre-loxP system, Dppa3-Cre mice were mated with Rosa26-LSL-METRNL knock-in mice（R26-LSL-METRNL+/-）to generate R26-L-METRNL+/- mice. The genotypes of the offsprings were identified, and tissues of the blood, heart, liver, spleen, lung, kidney, brain, white adipose and muscle were collected. The expression of human METRNL gene in mice was investigated by quantitative real-time PCR, western blot and enzyme linked immunosorbent assay. Results Compared with wild type control mice, human METRNL in R26-L-METRNL+/- mice significantly expressed at both mRNA and protein levels in tissues, with abundant METRNL protein in blood. Conclusion The mouse model overexpressing human METRNL gene（R26-L-METRNL+/- mouse）was successfully constructed. |
| keywords:METRNL systemic overexpression mouse |
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