| 基于解吸电喷雾质谱成像技术分析番红花球茎中代谢物的组织分布 |
| 投稿时间：2023-10-30 修订日期：2024-01-30 点此下载全文 |
| 引用本文：陈希,谢星光,冯坤苗,陆颖洁,韩婷.基于解吸电喷雾质谱成像技术分析番红花球茎中代谢物的组织分布[J].药学实践杂志,2024,42(4):169~172,180 |
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| 基金项目:国家自然科学基金面上项目（82174091）；上海市崇明区“可持续发展科技创新行动计划”（CK2021-03） |
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| 中文摘要:目的 探究不同产地番红花球茎中内源性代谢物的分布特征。方法 利用解吸电喷雾（DESI）质谱成像技术，通过优化样品前处理，建立了一种对番红花球茎内源性代谢物可视化分析的方法。结果 实现了黄酮、有机酸、氨基酸、类胡萝卜素和环烯醚萜苷等代谢物的原位表征；L-瓜氨酸、苯乙酰甘氨酸、紫苜蓿酚和栀子苷等特异性分布在球茎中；芹菜素7-（6''-O-乙酰基）-葡萄糖苷、异鼠李素-3-O-葡萄糖苷、蜀黍氰苷6'-葡萄糖苷、芹菜素7-O-二葡糖醛酸主要分布在顶芽中；对于在球茎部位分布的化合物，产于上海的番红花球茎丰度最高，浙江次之，安徽最低。结论 番红花球茎在不同产地及同一产地不同部位的代谢物分布存在显著差异，黄酮和黄酮衍生物如异鼠李素-3-O-葡萄糖苷、芹菜素衍生物主要分布于顶芽中，此外，天然植物保护剂蜀黍氰苷6'-葡萄糖苷也主要在顶芽分布，而作为能量和物质供应的氨基酸则主要积累在球茎。 |
| 中文关键词:番红花球茎 解吸电喷雾质谱成像 不同产地 不同部位 代谢物分布 |
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| Analysis of tissue distribution of metabolites in Crocus sativus L. corms based on DESI mass spectrometry imaging technique |
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| Abstract:Objective To explore the distribution characteristics of endogenous metabolites in Crocus sativus L. corms from different origins. Methods A method based on desorption electrospray ionization mass spectrometry imaging and optimized sample pretreatment was developed for directly visualize metabolites in C. sativus corms. Results In situ characterization of metabolites such as flavonoids, organic acids, amino acids, carotenoids, and cyclic enol ether terpene glycosides was achieved. L-Citruline, phenylacetylglycine, sativol, and geniposide were specifically distributed in the corms. Apigenin 7-（6''-O-acetyl）-glucoside, isorhamnetin-3-O-β-D-Glucoside, dhurrin 6'-glucoside, and Apigenin 7-O-diglucuronide were mainly distributed in the terminal bud. For compounds distributed in the corms, the highest abundance was found in corms from Shanghai, followed by Zhejiang and the lowest from Anhui. Conclusion The distribution of metabolites in different parts of C. sativus corms from different origins and the same origin varies significantly. Flavonoids and flavonoid derivatives such as isorhamnetin-3-O-β-D-Glucoside and apigenin derivatives are mainly distributed in the terminal buds, in addition, the natural plant protection agent dhurrin 6'-glucoside is also mainly distributed in the terminal corms, whereas amino acids, which are used as energy and material supplies, are mainly accumulated in the corms. |
| keywords:Crocus sativus L. corms desorption electrospray ionization mass spectrometry imaging different origins different parts of C. sativus corms metabolite distribution |
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