| 蓝草类药材基原植物叶片表皮显微结构研究 |
| 投稿时间：2024-04-19 修订日期：2024-11-13 点此下载全文 |
| 引用本文：白云俊,赵玉洋,金艳,付璐,袁媛.蓝草类药材基原植物叶片表皮显微结构研究[J].药学实践杂志,2025,43(4):174~179 |
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| 基金项目:国家重点研发计划（2022YFC3500901）；国家自然基金面上项目（82274053）；中国中医科学院科技创新工程项目（CI2023E002，CI2021A04001） |
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| 中文摘要:目的 蓝草类药材复杂的基原演变历程可能导致混伪品的出现，影响临床用药的准确性。该文为叶片入药的蓝草类药材提供叶表皮显微结构鉴定依据。方法 系统整理蓝草类药材所涉及的物种及其近缘种（4属10个物种），利用光学显微镜和扫描电子显微镜对其叶表皮显微结构进行观察分析，建立了基于显微结构的物种检索表。结果 综合气孔器分布位置、副卫细胞类型、气孔指数、气孔密度、表皮细胞垂周壁形态以及表皮附属物等特征可准确区分大青属大青，蓼属蓼蓝，菘蓝属菘蓝、宽翅菘蓝、三肋菘蓝和小果菘蓝，马蓝属翅柄马蓝、曲枝假蓝、球花马蓝和板蓝等物种。结论 叶表皮显微特征可准确区分蓝草类叶类药材的基原。 |
| 中文关键词:蓝草类药材 叶片 显微结构 物种鉴定 |
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| Study on leaf epidermal microstructure of medicinal blue herbs |
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| Abstract:Objective The complex evolutionary history of the origin of medicinal blue herbs might result in the presence of adulterants, affecting the accuracy and safety of clinical medication. To provide a reference basis for the identification of medicinal blue herbs with leaves as the medicinal part, based on the leaf epidermis microstructure. Methods The species belonging to medicinal blue herbs and their close relatives (10 species from 4 genera) were systematically investigated. The leaf epidermis microstructure of these species was observed and analyzed by optical microscopy and scanning electron microscopy. A species retrieval table was established based on the microstructure characteristics. Results By combining the distribution of stomata, types of subsidiary cells, stomatal index, stomatal density, characteristics of the periclinal walls of epidermal cells, and epidermal appendages, the species Clerodendrum cyrtophyllum, Polygonum tinctorium, Isatis indigotica, I. violascens, I. costata, I. minima, Strobilanthes wallichii, S. dalzielii, S. pentstemonoides, and S. cusia can be accurately distinguished. Conclusion Microscopic characteristics of leaf epidermis can provide reference data for accurately differentiating the botanical origins of medicinal blue herbs. |
| keywords:medicinal blue herbs leaf microstructure species identification |
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