| 高效液相色谱法同时测定纯阳正气胶囊中3种成分的含量 |
| 投稿时间：2022-01-17 修订日期：2022-06-21 点此下载全文 |
| 引用本文：文艺,蒙露露,王宝艺,韦飞雪,邓鹏,柳荣英.高效液相色谱法同时测定纯阳正气胶囊中3种成分的含量[J].药学实践杂志,2024,42(2):78~81 |
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| 基金项目:广西科技重大专项（桂科AA17292009）；桂林市“1020”创新驱动计划重大攻关（20170205） |
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| 中文摘要:目的 采用高效液相色谱法建立同时测定纯阳正气胶囊中橙皮苷、桂皮醛和丁香酚含量的方法。方法 色谱柱为Agilent PorosheⅡ 120 EC-C18（4.6 mm×150 mm，4 μm），以乙腈-水为流动相梯度洗脱，柱温35 ℃，流速1.0 ml/min，检测波长284 nm。结果 方法学验证表明，橙皮苷、桂皮醛和丁香酚3种成分线性关系良好（r≥0.999 9），精密度小于2.0%，平均加样回收率在98.0%~101.9%之间，稳定性和重复性的RSD均<3.0%，符合方法学要求。结论 该方法简便、稳定、重复性好、准确可靠，可用于纯阳正气胶囊的质量控制。 |
| 中文关键词:纯阳正气胶囊 橙皮苷 桂皮醛 丁香酚 含量测定 |
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| Simultaneous determination of three constituents in Chunyang Zhengqi capsules by HPLC |
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| Abstract:Objective To establish method for simultaneous determination of hesperidin, cinnamaldehyde and eugenol in Chunyang Zhengqi capsules by high performance liquid chromatography. Methods The column was Agilent PorosheⅡ 120 EC-C18 (4.6 mm×150 mm, 4 μm). The mobile phase was acetonitrile-water with gradient elution. The column temperature was 35℃. The flow rate was 1.0 ml/min, and the detection wavelength was 284 nm. Results The methodological verification showed that hesperidin, cinnamaldehyde and eugenol had a good linearity (r≥0.999 9). The precisions were less than 2.0%. The average recovery was between 98.0% and 101.9%. The stability and repeatability of RSD were also less than 3.0%, which met the requirements of method validation. Conclusion The method is simple, stable, reproducible and accurate, which could be used to the quality control of Chunyang Zhengqi capsules. |
| keywords:Chunyang Zhengqi capsules hesperidin cinnamaldehyde eugenol content determination |
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