| 表面增强拉曼光谱法快速测定尿液中曲马多的研究 |
| 投稿时间：2024-01-31 修订日期：2024-06-27 点此下载全文 |
| 引用本文：姚小静,计佩影,陆峰,施国荣,傅翔.表面增强拉曼光谱法快速测定尿液中曲马多的研究[J].药学实践杂志,2025,43(4):185~189 |
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| 基金项目:杨浦区科学技术委员会杨浦区卫生健康委员会科研课题（面上）(YPM202108)；杨浦区科学技术委员会杨浦区卫生健康委员会科研课题（青年）(YPQ202114） |
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| 中文摘要:目的 应用液液萃取（LLE）-表面增强拉曼光谱（SERS），建立快速检测尿液中曲马多的方法。方法 以氯仿∶异丙醇（9∶1）萃取剂从尿液中提取曲马多，采用增强拉曼光谱（波长为785 nm）检测尿液样品中的曲马多。结果 曲马多的定量曲线Y=204.35 X-465.62，r=0.9952，线性范围为1～100 μg/ml，该方法曲马多的检测限（S/N=3）为0.53 μg/ml；与常规方法相比，SERS具有高灵敏度及合理的重现性。结论 此方法简单、高效、经济，可以用于曲马多个性化用药的定性和定量分析。 |
| 中文关键词:曲马多 尿液 表面增强拉曼光谱 潜在不适当用药 |
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| Rapid determination of tramadol in urine by surface-enhanced Raman spectroscopy |
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| Abstract:Objective To establish a method for rapid detection of tramadol in urine by liquid-liquid extraction（LLE）-surface-enhanced Raman spectroscopy （SERS）.Methods Tramadol was extracted from urine with chloroform∶isopropyl alcohol （9∶1） extractant and detected in urine samples by enhanced Raman spectroscopy （wavelength 785 nm）. Results The quantitative curve of tramadol was Y=204.35 X-465.62, r=0.9952, and the linear range was 1-100 μg/ml. The detection limit of tramadol by this method （S/N=3） was 0.53 μg/ml. The sensitivity of SERS was higher than that of conventional methods, and it had reasonable reproducibility. Conclusion This method is simple, efficient and economical, and can be used for qualitative and quantitative analysis of tramadol personalized medicine. |
| keywords:tramadol urine surface-enhanced Raman spectroscopy potential inappropriate medication |
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