| 参麻颈复方对脑缺血损伤小鼠的脑保护作用及其机制研究 |
| 投稿时间：2022-08-16 修订日期：2023-03-21 点此下载全文 |
| 引用本文：刘景雪,何瑞华,陶霞,黄瑾.参麻颈复方对脑缺血损伤小鼠的脑保护作用及其机制研究[J].药学实践杂志,2023,41(11):666~671,685 |
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| 作者 | 单位 | E-mail | | 刘景雪 | 上海中医药大学附属岳阳中西医结合医院药剂科, 上海, 200437
海军军医大学第二附属医院药剂科, 上海, 200003 | | | 何瑞华 | 上海中医药大学附属岳阳中西医结合医院药剂科, 上海, 200437 | | | 陶霞 | 海军军医大学第二附属医院药剂科, 上海, 200003 | | | 黄瑾 | 上海中医药大学附属岳阳中西医结合医院药剂科, 上海, 200437 | john70550@163.com |
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| 基金项目:上海中医药大学附属岳阳中西医结合医院院级重点资助项目（2019YYZ07）；上海市进一步加快中医药传承创新发展三年行动计划（ZY（2021-2023）-0203-03）；上海申康医院发展中心临床科技创新项目（SHDC12021635） |
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| 中文摘要:目的 观察参麻颈复方颗粒对脑缺血损伤小鼠脑组织的保护作用，并探讨其可能机制。 方法 将SPF级C57 BL/6雄性小鼠30只随机分为模型对照组、参麻颈组、尼莫地平组，采用电凝法制备小鼠脑缺血损伤动物模型，给参麻颈组、尼莫地平组小鼠灌胃14 d，应用TTC染色法检测各组小鼠脑梗死体积，提取骨髓内皮祖细胞并测定细胞功能，用蛋白免疫印迹法（Western blot）检测内皮祖细胞中蛋白表达水平。结果 与模型对照组比较,参麻颈组小鼠脑梗死体积明显减少，其内皮祖细胞迁移、黏附以及形成小管的能力显著改善，内皮祖细胞中BDNF蛋白表达水平明显升高。结论 参麻颈复方颗粒对脑缺血损伤小鼠脑组织具有保护作用，可能与调节内皮祖细胞中BDNF的表达，改善骨髓来源内皮祖细胞功能密切相关。 |
| 中文关键词:参麻颈复方 脑缺血损伤 内皮祖细胞 神经功能 |
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| Protective effect of Shenmajing formula on ischemic brain injury in mice |
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| Abstract:Objective To observe the protective effect of Shenmajing formula on brain tissue of mice with cerebral ischemic injury and explore the possible mechanism. Methods Thirty SPF-grade C57 BL/6 male mice were randomly divided into model control group, Shenmajing group and nimodipine group, and the animal models of cerebral ischemic injury in mice were prepared by electrocoagulation. The protein expression level in endothelial progenitor cells were detected by Western blot. Results Compared with the model control group, the infarct volume of mice in the Shenmajing group was significantly reduced, and the migration, adhesion and tubule formation ability of endothelial progenitor cells were significantly improved, and the expression level of BDNF protein in endothelial progenitor cells was significantly increased. Conclusion The protective effect of Shenmajing granules on brain tissue of mice with cerebral ischemic injury could be closely related to the regulation of BDNF expression in endothelial progenitor cells and improvement of endothelial progenitor cell function of bone marrow origin. |
| keywords:Shenmajing formula cerebral ischemic injury endothelial progenitor cells neurological function |
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