| 河豚毒素处方前初步研究 |
| 投稿时间：2023-04-14 修订日期：2023-08-14 点此下载全文 |
| 引用本文：徐己人,郑紫云,李园,鲁莹,储智勇.河豚毒素处方前初步研究[J].药学实践杂志,2023,41(9):544~546,551 |
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| 中文摘要:目的 考察河豚毒素（TTX）在不同溶剂中的溶解性及稳定性，以及温度和pH值对稳定性的影响。方法 配制TTX不同介质的溶液，采用高效液相色谱法（HPLC）测定其在不同温度、不同pH缓冲液中的浓度，分析计算其溶解度及稳定性。结果 TTX在pH值为3.5时溶解度最大，随着pH值增加其溶解度逐渐降低。TTX在强碱条件下降解最为迅速，在70 ℃条件下、0.1 mol/L的氢氧化钠溶液中，20 min即完全降解。稳定性试验结果同样证明TTX在碱性条件下的稳定性最差，在37 ℃、pH值=7.4的磷酸缓冲盐溶液（PBS）中，TTX浓度在1~10 h时开始持续降低，28天降解率为88.07±0.27%。结论 TTX易溶于pH值=3.5的酸性水溶液，几乎不溶于碱性水溶液。其稳定性与温度、介质pH值密切相关，在酸性水溶液中较为稳定，在碱性条件下易降解，温度升高会加速其降解过程。 |
| 中文关键词:河豚毒素 HPLC 稳定性 溶解度 |
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| Preliminary pre-prescription study of Tetrodotoxin |
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| Abstract:Objective To investigate the solubility and stability of Tetrodotoxin (TTX) in different solvents, and the effect of temperature and pH on its stability. Method Solutions of TTX in different matrices were prepared. Their concentrations at different temperatures and pH buffers were determined by high performance liquid chromatography and their solubility and stability were analyzed and calculated. Result TTX was most soluble at pH 3.5 and its solubility decreased as the pH increased. TTX degraded most rapidly under strong alkali conditions, with complete degradation after 20 min of reaction at 0.1 mol/L sodium hydroxide and 70 ℃. The stability test results similarly demonstrated that TTX was least stable under alkaline conditions. In a PBS buffered solution at 37 ℃, pH 7.4, TTX concentration began to decrease consistently at 1~10h, with a degradation rate of 88.07±0.27% after 28 days. Conclusion TTX is readily soluble in acidic aqueous solutions at pH 3.5 and almost insoluble in alkaline aqueous solutions. Its stability is closely related to the temperature and pH of the medium. It is more stable in acidic aqueous solutions and easily degrades under alkaline conditions, and its degradation process could be accelerated by increasing temperature. |
| keywords:Tetrodotoxin HPLC Stability Solubility |
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