| 金线莲内生真菌Aspergillus sp. J218的次生代谢产物研究 |
| 投稿时间：2022-08-16 修订日期：2023-01-09 点此下载全文 |
| 引用本文：何旭辉,李祥昂,陈美言,王一丁,吴睿,郑承剑.金线莲内生真菌Aspergillus sp. J218的次生代谢产物研究[J].药学实践杂志,2023,41(7):411~415,442 |
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| 基金项目:国家自然科学基金资助项目（82174081）；上海市浦江人才计划（21PJD082） |
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| 中文摘要:目的 研究从金线莲Anectochilus roxburhii中分离的一株曲霉属内生真菌Aspergillus sp. J218的次生代谢产物。方法 利用Sephadex LH-20凝胶、硅胶和高效液相制备等色谱法从J218固体发酵产物的乙酸乙酯部位分离化合物，采用波谱方法对化合物进行结构鉴定。结果 从金线莲内生真菌Aspergillus sp. J218的次生代谢产物中共分离得到10个化合物，分别鉴定为kotanin（1）、flavasperone（2）、aurasperone B（3）、fonsecinone B（4）、fonsecinone D（5）、tensidol A（6）、fonsecinone A（7）、fonsecinone C（8）、aurasperone A（9）和fonsecinone F（10）。结论 从金线莲内生真菌曲霉属Aspergillus sp J218次生代谢产物分离鉴定多为二聚萘并吡喃酮类化合物，提示该菌株含有丰富的二聚化合成酶，可为后续探索该菌株中二聚萘并吡喃酮类化合物的生物合成途径提供线索。 |
| 中文关键词:曲霉属 金线莲 内生真菌 次生代谢产物 二聚萘并吡喃酮类化合物 |
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| Secondary metabolites of endophytic fungus Aspergillus sp. J218 from Anectochilus roxburhii |
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| Abstract:Objective To isolate the secondary metabolites of endophytic fungus Aspergillus sp. J218 from Anectochilus roxburhii. Methods Different chromatographic methods, including Sephadex LH-20 and silica gel chromatography as well as HPLC, were used to isolate compounds from the EtOAc fraction of the solid fermentation of J218, and their structures were identified by spectral methods. Results Ten compounds were isolated from the fermentation of J218 and their structures were individually identified as kotanin（1）, flavasperone（2）, aurasperone B（3）, fonsecinone B（4）, fonsecinone D（5）, ensidol A（6）, fonsecinone A（7）, fonsecinone C（8）, aurasperone A（9）, and fonsecinone F（10）. Conclusion Most compounds isolated from endophytic fungus Aspergillus sp. J218 in Anectochilus roxburhii were identified as dimeric naphthopyrones. These results suggest that this strain contains rich dimerization synthase, which could provide clues for the further exploration of the rational biosynthesis pathway of dimeric naphthopyrones in this strain. |
| keywords:Aspergillus sp. J218 Anectochilus roxburhii endophytic fungi secondary metabolites dimeric naphthopyrones |
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