| 质谱导向的肾指海绵Reniochalina sp.中环肽类成分研究 |
| 投稿时间：2022-06-11 修订日期：2023-02-16 点此下载全文 |
| 引用本文：吴宗梅,吴莹,张帅帅,孔灿,王淑萍,林厚文.质谱导向的肾指海绵Reniochalina sp.中环肽类成分研究[J].药学实践杂志,2023,41(5):316~320 |
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| 基金项目:国家自然科学基金项目（82173730，22137006） |
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| 中文摘要:目的 以质谱为导向对肾指海绵Reniochalina sp.中的环肽类成分进行研究。方法 采用质谱引导的程序性分离手段定向追踪并分离纯化海绵中的环肽类成分；通过理化常数测定、波谱数据比对确定化合物结构；利用CCK-8法对化合物进行初步细胞毒活性评价。结果 从肾指海绵Reniochalina sp.中分离获得3个环肽类化合物，分别鉴定为stylopeptide 1 (1)、hymenamide D (2)、axinastatin 2 (3)。化合物1对6种人肿瘤细胞株具有细胞毒性，IC50值范围为6.09 ~ 17.26 μmol/L。结论 化合物1 ~ 3首次分离自Reniochalina属海绵，化合物1是细胞毒性环七肽。 |
| 中文关键词:肾指海绵 化学成分 环肽 结构鉴定 |
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| Mass spectrometry-guided study on cyclic peptides from sponge Reniochalina sp. |
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| Abstract:Objective To study the cyclic peptides from sponge Reniochalina sp. under the guidance of mass spectrometry. Methods Mass spectrometry-guided procedural separation methods were used to track and isolate the cyclic peptides from the sponge genus Reniochalina. The structures of compounds were elucidated by the determination of physicochemical parameters and comparison of spectroscopic data. The preliminary cytotoxic activity of compounds was assessed by the Cell Counting Kit-8 (CCK-8) method. Results Three cyclic peptides were isolated from the sponge Reniochalina sp. and identified as stylopeptide 1 (1), hymenamide D (2) and axinastatin 2 (3). Compound 1 exhibited cytotoxicity against six human cancer cell lines with IC50 values ranging from 6.09 to 17.26 μmol/L. Conclusion Compound 1 - 3 were isolated from Reniochalina sp. for the first time, and compound 1 was a cytotoxic cyclic heptapeptide. |
| keywords:Reniochalina sp. chemical constituent cyclic peptide structure identification |
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