| 虎杖清脉饮对慢性脑缺血小鼠认知功能及肠道菌群的影响 |
| 投稿时间：2022-08-28 修订日期：2023-03-20 点此下载全文 |
| 引用本文：郭羽晨,丁华敏,邓珊珊,章越凡,李铁军.虎杖清脉饮对慢性脑缺血小鼠认知功能及肠道菌群的影响[J].药学实践杂志,2023,41(5):302~309 |
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| 基金项目:上海市浦东新区卫生系统重点专科建设资助（居家药学，PWZzK2002-08） |
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| 中文摘要:目的 从肠道菌群角度探讨虎杖清脉饮对慢性脑缺血小鼠认知功能改善的作用机制。方法 放置微弹簧圈于双侧颈总动脉致狭窄制备慢性脑缺血小鼠模型。灌胃给药12 w后，采用Morris水迷宫和LFB染色测定小鼠的认知功能和白质的损伤程度；提取各组小鼠盲肠内容物，进行16S rRNA基因V3+V4去扩增子信息采集与分析。结果 水迷宫实验结果显示，虎杖清脉饮组小鼠逃避潜伏期显著缩短，跨越平台次数和目标象限百分比增加，学习记忆功能明显改善。LFB染色显示，模型组白质损伤严重；虎杖清脉饮组白质损伤程度较轻。16S rRNA测序结果显示，与模型组相比，虎杖清脉饮组小鼠肠道菌群中疣微菌门、阿克曼菌属和丹毒荚膜菌属的丰度显著降低（P<0.05），而嗜木聚糖真杆菌属和异杆菌属的丰度显著增加（P<0.05）。结论 虎杖清脉饮具有改善慢性脑缺血小鼠认知功能的作用，其保护作用可能与调节慢性脑缺血小鼠肠道菌群有关。 |
| 中文关键词:虎杖清脉饮 慢性脑缺血 认知功能 16S rRNA 肠道菌群 |
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| Effects of Huzhang Qingmai decoction on cognitive function and intestinal flora in mice with chronic cerebral ischemia |
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| Abstract:Objective To investigate the mechanism of action of Huzhang Qingmai decoction (HZQMY) on the improvement of cognitive function in mice with chronic cerebral ischemia from the perspective of intestinal flora. Methods A mouse model of chronic cerebral ischemia was established by placing microcoils around the bilateral common carotid arteries to induce bilateral carotid artery stenosis (BCAS). After 12 weeks of intragastric administration, the cognitive function of the mice was measured by the Morris water maze; the myelin damage was analyzed by LFB staining; The contents of the cecum of the mice in each group were extracted and analyzed by 16S rRNA sequencing. Results The results of the water maze experiment showed that the mice in the HZQMY group had a significantly shorter escape latency, increased the number of crossings platform and the percentage of target quadrants. LFB staining showed that the white matter damage in the model group was severe; the white matter damage in the HZQMY group was milder. The results of 16S rRNA sequencing showed that compared with the model group, the abundance of Verrucomicrobiota, Akkermansia, and ErysiPelatoclostridium capsulatum in the intestinal flora in HZQMY group was significantly reduced (P<0.05), while the abundances of Eubacterium_xylanoPhilum and Allobaculum were significantly increased (P<0.05). Conclusion The protective effect of HZQMY, which has the effect of improving cognitive function in mice with chronic cerebral ischemia, may be related to the regulation of intestinal flora in mice with chronic cerebral ischemia. |
| keywords:HZQMY chronic cerebral ischemia cognitive function 16S rRNA intestinal flora |
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