| 一种中药组合物对ANIT诱导的小鼠胆汁淤积肝损伤的保护作用研究 |
| 投稿时间：2023-05-06 修订日期：2024-01-19 点此下载全文 |
| 引用本文：杨念,张博乐,张俊霞,张振强.一种中药组合物对ANIT诱导的小鼠胆汁淤积肝损伤的保护作用研究[J].药学实践杂志,2024,42(12):508~511,519 |
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| 基金项目:河南省科技攻关规划项目( 232102310473) ；河南中医药大学科研苗圃工程项目（MP2022-17） |
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| 中文摘要:目的 探讨一种中药组合物对α-萘异硫氰酸酯（ANIT）诱导的小鼠胆汁淤积肝损伤的保护作用。方法 取8周龄SPF级昆明小鼠，随机分成6组，每组10只，分别为空白对照组（灌胃生理盐水），模型对照组（灌胃生理盐水）,中药组合物低（灌胃剂量5.25 g/kg）、中（灌胃剂量11.25 g/kg）、高（灌胃剂量20.25 g/kg）剂量组，阳性对照组（熊去氧胆酸，0.1 g/kg），每日1次，连续灌胃药7 d。第5天，空白对照组灌胃给予等量橄榄油，其他各组灌胃ANIT（65 mg/kg）造模。造模48 h后，各组小鼠末次给药后30 min，收集小鼠血液和肝组织样本，进行各项指标的检测。采用试剂盒检测血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、总胆汁酸（TBA）、γ-谷氨酰转肽酶（γ-GT）、总胆红素（TBIL）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）和丙二醛（MDA）含量。采用HE染色观察小鼠肝脏病理学变化。结果 与模型组相比，中药组合物各剂量组能够明显降低小鼠外周血清中ALT、AST、ALP、TBA、TBTL、γ-GT和MDA含量（P<0.05），显著提高SOD和GSH-PX水平（P<0.05）。与模型组相比，中药组合物低剂量组、高剂量组肝细胞脂肪变性减少，炎症浸润减轻；中剂量组肝脏细胞脂肪变性显著减少，炎症浸润现象明显改善，肝细胞呈索状排列，肝小叶结构完整。结论 中药组合物能够改善肝损伤血清生化学指标、降低黄疸和清除氧化自由基，从而发挥对ANIT诱导的小鼠胆汁淤积性肝损伤的保护作用。 |
| 中文关键词:中药组合物 胆汁淤积 生化学指标 病理变化 |
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| The protective effect of a traditional chinese medicine composition on ANIT induced liver injury in mice with cholestasis |
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| Abstract:Objective To explore the protective effect of a traditional chinese medicine composition on ANIT induced cholestasis liver injury in mice. Methods 8-week old SPF Kunming mice were randomly divided into 6 groups, 10 mice in each group which were the blank control group （normal saline gavage）, the model control group （normal saline gavage）, the low （gavage dose: 5.25 g/kg） group, the medium （gavage dose: 11.25 g/kg） group and the high （gavage dose: 20.25 g/kg） group of traditional chinese medicine composition, the positive control （Ursodeoxycholic acid, UDCA, 0.1 g/kg）. Mice were administered with continuous gavage once a day, for 7 consecutive days. On the 5th day, the blank normal control group was given an equal amount of olive oil by gavage, all other groups were given ANIT （65 mg/kg） by gavage for modeling. After 48 hours of modeling, 30 minutes after the last administration of each group of mice, blood and liver tissue samples were collected for testing of various indicators. The corresponding kits were used to detect the content of alanine transaminase （ALT）, aspartate transaminase （AST）, alkaline phosphatase （ALP）, total bile acid （TBA）, γ-glutamyl transpeptidase （γ-GT）, total bilirubin （TBIL）, superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX） and malondialdehyde （MDA） in peripheral blood of mice, respectively. HE staining was used to observe the pathological changes in the liver of mice. Results Compared with the model group, each dose group of traditional chinese medicine composition reduced the levels of ALT, AST, ALP, TBA, TBIL, γ-GT and MDA （P<0.05） significantly, markedly increased the levels of SOD and GSH-PX （P<0.05）. Compared with the model group, the low-dose and high-dose groups of traditional chinese medicine composition showed a decrease in hepatic steatosis and a reduction in inflammatory. The moderate dose group showed a significant reduction in hepatic cell steatosis and a significant improvement in inflammatory infiltration, the liver cells were arranged in cords, and the structure of lobules of liver was intact. Conclusion Traditional chinese medicine composition could improve serum biochemical indicators of liver injury, reduce jaundice and eliminate oxidative free radicals, thereby exerting a protective effect against ANIT-induced cholestatic liver injury in mice. |
| keywords:Traditional chinese medicine composition Cholestasis Biochemical indexes Pathological changes |
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