| 河豚毒素缓释微球含量测定方法的建立 |
| 投稿时间：2022-08-13 修订日期：2023-02-02 点此下载全文 |
| 引用本文：王琦,卢光照,李园,樊莉,张翮,鲁莹.河豚毒素缓释微球含量测定方法的建立[J].药学实践杂志,2023,41(3):182~186 |
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| 基金项目:军队“双重”项目：生物毒素战剂控释研究与应用 |
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| 中文摘要:目的 建立测定河豚毒素（TTX）缓释微球中的含量检测方法。方法 采用HPLC法，色谱柱为Agilent Zorbax SB C18柱（4.6 mm×150 mm，5 μm）；流动相为5％乙腈-95％ 8 mmol/L庚烷磺酸钠溶液（0.005％TFA，pH4.0）；流速1.0 ml/min；紫外检测波长200 nm；柱温30 ℃。结果 该方法专属性好，TTX在1~20 μg/ml浓度范围内线性良好，该方法日内精密度、日间精密度、稳定性、重复性均符合要求，加样回收率范围为98.0%~102.0%。结论 本研究建立了适用于缓释微球中河豚毒素含量测定的HPLC方法，该方法准确可靠，专属性强，可以实现定量检测。 |
| 中文关键词:河豚毒素 高效液相色谱 缓释微球 庚烷磺酸钠 |
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| Establishment of determination of tetrodotoxin sustained-release microspheres |
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| Abstract:Objective To establish a detection method for the determination of tetrodotoxin (TTX) in sustained-release microspheres. Methods The HPLC separation of tetrodotoxin was performed on an Agilent ZORBAX SB-C18 column (4.6mm×150mm,5 μm) with acetonitrile, 8mmol/L sodium heptane sulfonate containing 0.005% TFA (5:95) (pH 4.0) as the mobile phase. The flow rate was 1.0 ml/min. The UV detection wavelength was 200 nm and the column temperature was 30 ℃. Results The method had good specificity and linearity of TTX in the concentration range of 1−20 μg/ml. The intra-day precision, inter-day precision, stability and repeatability of the method were good, and the average recoveries were found between 98.0% and 102.0%. Conclusion This study established an HPLC method which was suitable for the determination of tetrodotoxin sustained-release microspheres. The method is accurate and reliable within the applicable range, with strong specificity, which could lead to quantitative detection. |
| keywords:Tetrodotoxin HPLC sustained-release microspheres sodium heptane sulfonate |
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