| 响应面优化微波辅助绿茶茶多酚提取工艺 |
| 投稿时间：2022-05-06 修订日期：2022-10-29 点此下载全文 |
| 引用本文：马小雨,罗彩萍,刘悦.响应面优化微波辅助绿茶茶多酚提取工艺[J].药学实践杂志,2023,41(2):119~124,134 |
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| 基金项目:上海市科学技术委员会“启明星”项目(21QA1411600） |
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| 中文摘要:目的 优化微波辅助绿茶茶多酚的提取工艺。方法 建立没食子酸标准曲线，通过引入校正因子测定绿茶提取液中茶多酚浓度以计算茶多酚提取得率；重点研究微波提取时间、微波输出功率、料液比、乙醇体积分数4项单因素水平对茶多酚提取得率的影响，初步确定4项单因素水平的适宜范围，并采用响应面法进一步提高绿茶茶多酚提取工艺。结果 最佳提取工艺为：提取时间37 s、微波输出功率350 w、料液比1∶45 (g/ml)、乙醇体积分数55%，茶多酚实际提取得率为25.65%，与理论值相差不大。结论 响应面优化的微波辅助绿茶茶多酚提取工艺省时可行、提取得率较高。 |
| 中文关键词:绿茶 茶多酚 含量测定 响应面优化 提取工艺 |
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| Optimization of microwave-assisted extraction of green tea polyphenols by response surface methodology |
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| Abstract:Objective To optimize the microwave-assisted extraction process of green tea polyphenols. Methods The extraction yield of tea polyphenols was figured up by building the standard curve of gallic acid and examining the concentration of tea polyphenols in green tea extract with the introduction of a correction factor. The effects of four single factor levels of microwave extraction time, microwave output power, liquid-to-material yield, and ethanol volume fraction on the extraction yield of tea polyphenols were primarily studied in this experiment. The response surface was applied to further optimize the extraction process of green tea polyphenols after exploring the appropriate range of four single factor levels. Results The optimal extraction process was as follows: extraction time 37 s, microwave output power 350 w, material - liquid yield 1∶45 (g/ml), ethanol volume fraction 55%, and the actual extraction yield of tea polyphenols was 25.65%, which was not much different from the theoretical value. Conclusion The microwave-assisted green tea polyphenol extraction process optimized by response surface methodology is time-saving and practicable, and the extraction yield is high. |
| keywords:green tea tea polyphenols content determination response surface optimization extraction process |
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