| 夜宁胶囊对小鼠镇静催眠作用及其机制的研究 |
| 投稿时间：2022-11-16 修订日期：2024-01-30 点此下载全文 |
| 引用本文：李清,郭宜银,陈颖,瞿发林,董文燊,戈煜.夜宁胶囊对小鼠镇静催眠作用及其机制的研究[J].药学实践杂志,2024,42(8):346~349 |
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| 基金项目:军队医疗机构制剂标准提高科研专项课题（13ZJZ16-3） |
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| 中文摘要:目的 研究夜宁胶囊对小鼠的镇静催眠作用，并初步探究其作用机制。方法 将小鼠随机分成空白对照组，艾司唑仑组（0.8 mg/kg），夜宁胶囊低、中和高剂量组（400、600和800 mg/kg），通过开野实验，协同阈上和阈下剂量戊巴比妥钠对小鼠睡眠影响的实验，观察并记录夜宁胶囊对小鼠自主活动、入睡潜伏期、睡眠时长和入睡率的影响。用酶联免疫法检测小鼠脑组织中γ-氨基丁酸（GABA）、5-羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NE）的水平。结果 与空白对照组相比，夜宁胶囊中、高剂量组（P<0.05、P<0.01）能显著降低小鼠自主活动次数；夜宁胶囊中、高剂量组能显著缩短小鼠入睡潜伏期（P<0.05、P<0.05），并延长睡眠时间（P<0.05、P<0.01）；夜宁胶囊中、高剂量组（P<0.05、P<0.01）能显著增加小鼠的入睡率。与空白对照组相比，高剂量的夜宁胶囊可明显增加小鼠脑组织中GABA（P<0.05）、5-HT（P<0.05）、DA（P<0.05）、NE（P<0.01）含量。结论 夜宁胶囊具有良好的镇静催眠作用，其作用机制与提高脑内GABA、5-HT、DA和NE水平有关。 |
| 中文关键词:夜宁胶囊 镇静催眠 失眠症 戊巴比妥钠 神经递质 |
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| Sedative and hypnotic effects and mechanism of Yening Capsules on mice |
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| Abstract:Objective To study the sedative and hypnotic effects of Yening Capsules and investigate its bioactive mechanism in mice. Methods The mice were randomly divided into control group, estazolam group （0.8 mg/kg）, low, medium and high-dose Yening Capsules groups （400, 600 and 800 mg/kg）. The locomotor activity, latency to persistent sleep, sleep duration and sleep rate were determined respectively in mice via the open field test and injection of pentobarbital sodium in subthreshold and suprathreshold doses. The content of GABA, 5-HT, DA and NE in brain tissue of mice were detected by enzyme-linked immunosorbent assay（ELISA）. Results Compared with the control group, Yening Capsules medium and high dose group （P<0.05, P<0.01）significantly decreased the locomotor activity of mice.The sleep latency in Yening Capsules medium and high dose group were significantly shorten （P<0.05, P<0.05）and the sleep duration （P<0.05, P<0.01）were extended. The sleep rate of Yening Capsules medium and high dose groups （P<0.05, P<0.01） was significantly increased. Compared with the control group, high dose of Yening Capsules can significantly increase GABA (P<0.05), 5-HT (P<0.05), DA (P<0.05), NE (P<0.01) in mouse brain tissue. Conclusion Yening Capsules had obvious sedative and hypnotic effects, and its mechanism may be related to the increasement of GABA, 5-HT, DA and NE level in brain tissue of mice. |
| keywords:Yening Capsules sedative hypnosis insomnia pentobarbital sodium neurotransmitter |
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