| 虎奶菇菌核多糖功能化纳米硒抗疲劳功效研究 |
| 投稿时间：2022-06-16 修订日期：2022-10-14 点此下载全文 |
| 引用本文：邹思,吴岩斌,吴锦忠,吴建国,黄家兴.虎奶菇菌核多糖功能化纳米硒抗疲劳功效研究[J].药学实践杂志,2024,42(10):426~432 |
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| 基金项目:国家自然科学基金项目（81700524）；福建省自然科学基金面上项目（2022J01866）；福建中医药大学校管科研项目（X2021019-重点） |
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| 中文摘要:目的 通过检测小鼠后肢肌肉相对长度、负重游泳时间及其血清和肝脏相关指标，探究虎奶菇菌核多糖功能化纳米硒（PTR-SeNPs）的体内抗疲劳功效。方法 48只雄性C57/BL6小鼠分为4组，每组12只，即对照组、游泳训练组（EC组）、PTR-SeNPs低剂量组（LPTR-SeNPs组）、PTR-SeNPs高剂量组（HPTR-SeNPs组），分别给予生理盐水（对照组与EC组）、LPTR-SeNPs[2.5 μmol/(kg·bw)]和HPTR-SeNPs[10 μmol/(kg·bw)]，1次/d，连续灌胃21 d。通过磁共振成像系统分析PTR-SeNPs对小鼠游泳训练后肢肌肉结构的影响，同时测定小鼠的负重游泳时间并检测血清中血乳酸（BLA）、血尿素氮（BUN）、谷丙转氨酶（ALT）、谷草转氨酶（AST）和乳酸脱氢酶（LDH）含量及肝脏中肝糖原（HG）、丙二醛（MDA）水平和过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活力。结果 与对照组比较，EC组小鼠血清BLA、BUN、ALT、AST及LDH含量显著升高（P<0.05或P<0.01），HPTR-SeNPs组小鼠肝脏中CAT含量显著升高（P<0.01），小鼠后肢肌肉相对长度显著增长（P<0.05），负重力竭游泳时间提高（P<0.05），L/HPTR-SeNPs组MDA水平无明显差异；与EC组比较，HPTR-SeNPs组小鼠负重游泳时间显著延长（P<0.01），BLA及BUN含量均显著降低（P<0.05或P<0.01），L/HPTR-SeNPs组HG含量显著增加（P<0.05或P<0.01），HPTR-SeNPs组血清ALT、AST及LDH水平均显著降低（P<0.05或P<0.01），肝脏CAT活力显著升高（P<0.01），LPTR-SeNPs组血清AST活力显著降低（P<0.05）、肝脏SOD活力显著升高（P<0.05）。结论 PTR-SeNPs具有改善肝脏生理功能、增加糖原储备、减少代谢物堆积及增强机体抗氧化能力，以及减缓疲劳的作用，具有开发成保健品或药品的潜力。 |
| 中文关键词:虎奶菇多糖纳米硒 游泳训练 抗氧化应激 抗疲劳活性 |
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| Anti-fatigue activity of selenium nanoparticles functionalized by polysaccharides from Pleurotus tuber-regium sclerotium |
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| Abstract:Objective To investigate the anti-fatigue effect of PTR-SeNPs in vivo by measuring the muscle relative length of hindlimb, load-bearing swimming time as well as serum and liver indexes of mice. Methods 48 male C57/BL6 mice were randomly assigned into 4 groups with 12 mice in each group, including vehicle control group （control group）, swimming training exercise group （EC group） with vehicle treatment, swimming training exercise with low dose of PTR-SeNPs group （LPTR-SeNPs） and high dose of PTR-SeNPs group （HPTR-SeNPs）. The mice were intragastrically administrated with normal saline in both control group and EC group, as well as 2.5 and 10 μmol/（kg·bw） PTR-SeNPs in LHPTR-SeNPs group, respectively, once per day for consecutively 21 days. After swimming training exercise, the muscle structures in the hind limb of mice were examined by magnetic resonance imaging. Furthermore, the burdened swimming time was measured, the serum content of blood lactic acid （BLA）, urea nitrogen （BUN）, alanine aminotransferase （ALT）, glutamic oxalate aminotransferase （AST） and lactate dehydrogenase （LDH）, as well as the hepatic level of glycogen （HG）, malondialdehyde （MDA） and activity of catalase （CAT） and superoxide dismutase （SOD） were determined. Results Compared with the control group, the serum contents of BLA, BUN, ALT, AST and LDH in EC group （P<0.05 or P<0.01） and hepatic CAT in HPTR-SeNPs group （P<0.01） were significantly increased. The muscle relative length of hind limbs and the burdened swimming time were extended by HPTR-SeNPs markedly （P<0.05）. There was no significant difference in MDA level in LHPTR-SeNPs group. Compared with EC group, the burdened swimming time of mice was significantly prolonged （P<0.01）, the contents of BLA and BUN were obviously decreased in the HPTR-SeNPs group （P<0.05 or P<0.01）, the level of HG was significantly increased in the LHPTR-SeNPs groups （P<0.05 or P<0.01）, the serum content of ALT, AST and LDH were markedly decreased in the HPTR-SeNPs group （P<0.05 or P<0.01）. Hepatic SOD activity was remarkably increased in LPTR-SeNPs group （P<0.05）, the level of CAT was evidently increased （P<0.01） and AST was decreased （P<0.05） in the HPTR-SeNPs group. Conclusion PTR-SeNPs could improve the liver physiological function, increase glycogen storage, reduce the accumulation of metabolites and enhance the body’s antioxidant capacity to ameliorate fatigue significantly, which could present the potential to be developed into health care products or drugs. |
| keywords:PTR-SeNPs swimming training exercise antioxidant stress anti-fatigue effect |
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