| 黄精多糖对糖尿病小鼠降糖作用及肠道影响的研究 |
| 投稿时间：2022-06-14 修订日期：2022-09-22 点此下载全文 |
| 引用本文：任群利,张信群,王苗,李小兰,姚燕子,冉应会,王倩.黄精多糖对糖尿病小鼠降糖作用及肠道影响的研究[J].药学实践杂志,2022,40(6):510~514 |
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| 作者 | 单位 | E-mail | | 任群利 | 遵义医科大学口腔医学院, 贵州 遵义 563003
贵州省普通高等学校微生物资源与药物开发特色重点实验室, 贵州 遵义 563003 | | | 张信群 | 正安县人民医院, 贵州 遵义 563003 | | | 王苗 | 遵义医科大学口腔医学院, 贵州 遵义 563003
贵州省普通高等学校微生物资源与药物开发特色重点实验室, 贵州 遵义 563003 | | | 李小兰 | 遵义医科大学口腔医学院, 贵州 遵义 563003
贵州省普通高等学校微生物资源与药物开发特色重点实验室, 贵州 遵义 563003 | | | 姚燕子 | 遵义医科大学口腔医学院, 贵州 遵义 563003
贵州省普通高等学校口腔疾病研究特色重点实验室, 贵州 遵义563003 | | | 冉应会 | 遵义医科大学基础医学院, 贵州 遵义 563003 | | | 王倩 | 遵义医科大学口腔医学院, 贵州 遵义 563003
贵州省普通高等学校口腔疾病研究特色重点实验室, 贵州 遵义563003 | qianwang07@126.com |
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| 基金项目:遵义医学院硕士启动基金项目（F-904）；贵州省高层次创新型人才（CJ-990）；遵义市科技局计划项目（遵市科合HZ字（2020）60号、遵市科合HZ字（2020）55号）；遵义医学院优秀青年人才项目（17zy-002）；贵州省科技计划项目（黔科合平台人才[2018]5772-067、黔科合平台人才[2018]5772-062） |
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| 中文摘要:目的 研究黄精多糖对2型糖尿病小鼠降血糖作用及其对肠道菌群和小肠病理结构的影响。方法 雄性小鼠50只，除10只正常饲养外，其余以高脂高糖饲料喂养6周，腹腔注射链脲佐菌素制备2型糖尿病小鼠模型，建模成功后随机分为4组：模型组、黄精多糖高、中、低(500、250、125 mg/kg)组，模型组小鼠给予生理盐水。记录各组小鼠体重和血糖值变化。4周后，收集粪便，进行16S rRNA高通量测序，HE染色观察小肠组织病理结构变化。结果 小鼠在糖尿病状态下体重下降，给予黄精多糖高、中、低剂量后小鼠体重分别增加14.24%、11.97%和8.78%，血糖值分别降低26.6%、22.3%和13.3%。此外，小肠病理组织学排列错乱和肿胀现象有所改善。模型组和黄精多糖组之间的肠道微生物存在显著差异，模型组中疣微菌门显著增多，而健康组和黄精多糖灌胃组中厚壁菌门和拟杆菌门的微生物丰度更高。结论 黄精多糖对糖尿病小鼠具有显著的降血糖作用，且对其肠道具有一定保护作用，其机制可能通过增加有益菌丰富度，提高小鼠免疫功能来实现的，且呈一定剂量效应关系，其免疫功能有待进一步研究。 |
| 中文关键词:黄精多糖 糖尿病 降血糖 小鼠 肠道微生物 |
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| Study on hypoglycemic effect and intestinal effect of Polygonatum sibiricum polysaccharides in diabetic mice |
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| Abstract:Objective To study the hypoglycemic effect of Polygonatum sibiricum polysaccharides on type 2 diabetic mice and its effects on intestinal flora and pathological structure of small intestine. Methods Fifty male mice were used, except 10 were fed normally, the others were fed with high-fat and high-sugar diet for 6 weeks, and then injected with streptozotocin intraperitoneally to make type 2 diabetes mice model. After successful modeling, they were randomly divided into model group and Polygonatum sibiricum polysaccharides (500, 250, 125 mg/kg) group and the model group mice were given normal saline. The changes of bodyweight and blood glucose of mice in each group were recorded. After 4 weeks, feces were collected and sequenced by a 16S rRNA high-throughput sequencing, and the pathological changes of small intestine were observed by HE staining. Results In diabetic mice, the weight decreased. After given Polygonatum sibiricum polysaccharides, the weight of mice increased by 14.24%,11.97% and 8.78%, and the blood glucose decreased by 26.6%, 22.3% and 13.3%, respectively after high, medium and low doses of Polygonatum sibiricum polysaccharides were administered. In addition, the pathological disorder and swelling of intestinal histopathology were improved. There were significant differences in intestinal microorganisms between the model group and the Polygonatum sibiricum polysaccharides. Verrucomicrobiae in the model group increased significantly, while the microorganism abundance of Firmicutes and Bacteroidetes in the healthy group and Polygonatum sibiricum polysaccharides group was higher. Conclusion Polygonatum sibiricum polysaccharides has a significant hypoglycemic effect on diabetic mice and a certain protective effect on their intestines, the mechanism may be achieved by increasing the richness of beneficial bacteria and improving the immune function of mice, it’s in a certain dose-effect relationship, and its immune function needs further study. |
| keywords:Polygonatum sibiricum polysaccharides diabetes mellitus hypoglycemia mice intestinal microorganisms |
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