| 中药海龙抗D-半乳糖诱导衰老小鼠学习记忆损伤作用研究 |
| 投稿时间：2022-01-20 修订日期：2022-04-09 点此下载全文 |
| 引用本文：张警文,何旭辉,夏天爽,蒋益萍,辛海量.中药海龙抗D-半乳糖诱导衰老小鼠学习记忆损伤作用研究[J].药学实践杂志,2022,40(3):259~264 |
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| 基金项目:国家重点研发计划资助（2019YFC1711000） |
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| 中文摘要:目的 研究中药海龙对D-半乳糖诱导衰老小鼠学习记忆损伤的影响及其作用机制。方法 采用高效液相色谱（HPLC）技术测定海龙中抗学习记忆损伤有效成分二十二碳六烯酸（DHA）含量；腹腔注射D-半乳糖(D-gal)制备衰老小鼠模型，以Morris水迷宫实验及蛋白免疫印迹法等，对小鼠学习记忆能力、海马组织氧化损伤相关生化指标及蛋白表达进行检测，探究海龙对衰老小鼠学习记忆损伤的保护作用及其机制。结果 HPLC结果显示，海龙中DHA含量为7.761 3 mg/g（以生药计），约占总成分的47％；Morris水迷宫实验结果显示，海龙可减少学习记忆损伤小鼠逃避潜伏期时间，增加小鼠目标象限游泳时间、游泳路程占比、穿台次数，改善小鼠学习记忆损伤；此外，海龙可激活衰老小鼠海马组织AKT/FOXO1/SOD2信号通路，上调氧化应激通路相关蛋白表达，降低衰老小鼠海马组织氧化损伤程度改善小鼠学习记忆损伤。结论 本研究发现海龙富含二十二碳六烯酸，具有改善D-半乳糖诱导衰老小鼠学习记忆损伤的作用，并初步阐明其作用机制与抗氧化相关，为中药海龙抗学习记忆损伤研究提供了实验依据。 |
| 中文关键词:海龙 二十二碳六烯酸 D-半乳糖 学习记忆损伤 海马组织 氧化损伤 |
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| Effect of traditional Chinese medicine Syngnathus on D-galactose-induced learning and memory impairment in aging mice |
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| Abstract:Objective To study the effect of traditional Chinese medicine, Syngnathus on learning and memory impairment induced by D-galactose in aging mice and its mechanism of action.Methods HPLC was used to determine the content of DHA, the active ingredient in anti-learning and memory impairment in Syngnathus. The aging mouse model was prepared by intraperitoneal injection of D-galactose (D-gal). Morris water maze test and Western blot were used to detect the ability of learning and memory, biochemical indicators and protein expression related to oxidative damage in the hippocampus, and to explore the protective effect and mechanism of Syngnathus on learning and memory impairment in aging mice.Results HPLC results showed that the DHA content in Syngnathus was 7.761 3 mg/g (calculated as crude drug), accounting for about 47% of the total composition. Morris water maze results showed that Syngnathus could reduce the escape latency of learning and memory-impaired aging mice and increase the target quadrant swimming time, the proportion of swimming distance and the number of times of crossing the platform, and improve the learning and memory impairment of mice. In addition, Syngnathus can activate the AKT/FOXO1/SOD2 signaling pathway in the hippocampus of aging mice with learning and memory impairment, promote the expression of oxidative stress pathway-related proteins, and improve the learning and memory impairment in aging mice by reducing the degree of oxidative damage in the hippocampus of aging mice.Conclusion This study found that Syngnathus is rich in DHA, which has the effect of improving learning and memory impairment induced by D-galactose in aging mice, and preliminarily clarified that its mechanism of action is related to anti-oxidation. Experimental evidence is provided. |
| keywords:Syngnathus docosahexaenoic acid D-galactose learning and memory impairment hippocampus oxidative damage |
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