| 基于生物信息学技术探讨补骨脂致肝损伤的作用机制 |
| 投稿时间：2021-03-22 修订日期：2021-06-04 点此下载全文 |
| 引用本文：余丹,迟骁玮.基于生物信息学技术探讨补骨脂致肝损伤的作用机制[J].药学实践杂志,2022,40(3):254~258 |
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| 基金项目:高等学校博士学科点专项科研基金（20130096120012） |
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| 中文摘要:目的 基于生物信息学技术建立补骨脂成分-靶点-信号通路-肝损伤网络，探讨补骨脂导致肝损伤的作用机制。方法 通过TCMIP数据库收集补骨脂成分以及肝损伤有关的靶点，依据反向药效团匹配方法预测补骨脂成分的作用靶点；采用Cytoscape 构建补骨脂成分-靶点网络，对补骨脂作用靶点构建网络模型进行GO与KEGG富集分析。结果 补骨脂中有22个成分作用于31个肝损伤靶点蛋白，血清白蛋白（ALB）、谷胱甘肽S-转移酶P（GSTP1）、运甲状腺素蛋白（TTR）和过氧化物酶体增殖物激活受体γ（PPARG）可能是关键节点蛋白。KEGG分析遴选出化学物致癌、AMPK、PPAR信号、P450代谢和谷胱甘肽等信号通路。结论 补骨脂可能是作用于ALB、GSTP1、TTR与PPARG等靶点并调控五类信号通路导致肝损伤。 |
| 中文关键词:补骨脂 生物信息学 肝损伤 |
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| Mechanism of Psoralea corylifolia Linn on liver injury based on bioinformatics |
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| Abstract:Objective To explore the mechanism of Psoralea corylifolia Linn (PCL) on liver injury by establishing the biological function and pathway network of PCL components, targets and protein interactions based on bioinformatics. Methods The components of PCL and potential liver-injury related targets were collected from TCMIP database. The targets of PCL were predicted by the reverse pharmacophore matching method. Cytoscape software was applied for the construction of active components-targets network map. Protein-protein interaction network was constructed by STRING database. Gene ontology functional enrichment analysis and KEGG pathway enrichment analysis were conducted to predict the liver injury mechanism of PCL. Results 22 components were identified from PCL with the corresponding 31 potential liver injury targets, mainly on serum albumin (ALB), glutathione S-transferase P (GSTP1), transthyretin (TTR) and peroxisome proliferator activated receptor gamma (PPARG) by PPI network analysis. The chemical carcinogenesis, adenosine 5 '- monophosphate activated protein kinase (AMPK) signal, PPAR signal, liver enzyme P450 and its harmful substance metabolism, glutathione metabolism and other signaling pathways were selected by KEGG analysis. Conclusion The active components of PCL may target on ALB, GSTP1, TTR and PPARG to regulate AMPK and PPAR signaling pathways, leading to liver injury. |
| keywords:Psoralea corylifolia Linn bioinformatics liver injury |
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