| 槟榔多酚提取物中总多酚和儿茶素类的含量测定 |
| 投稿时间：2021-06-03 修订日期：2022-04-26 点此下载全文 |
| 引用本文：马江红,王荣,杜兴,赵安鹏,王子晗,张浩波.槟榔多酚提取物中总多酚和儿茶素类的含量测定[J].药学实践杂志,2022,40(3):243~247 |
| 摘要点击次数: 879 |
| 全文下载次数: 1148 |
|
| 基金项目:国家自然科学基金项目（81673508)；2020年中央高校创新团队项目（3192020009） |
|
| 中文摘要:目的 建立槟榔多酚提取物中总多酚和儿茶素类含量的测定方法，为槟榔多酚提取物的质量控制提供参考。方法 采用紫外分光光度法对槟榔提取物中总多酚的含量进行测定；高效液相色谱法对儿茶素类物质进行含量测定。结果 紫外分光光度法测定槟榔提取物中总多酚含量在9.8~58.8 μg/ml范围内线性关系良好，平均加样回收率为98.44%，RSD为1.4%；在高效液相色谱测定中，儿茶素、表儿茶素和原儿茶酸3种主要成分完全分离，在各自范围内线性关系良好，平均加样回收率为99.17%~101.67%，RSD为1.2%~2.5%。结论 建立的含量测定方法简单、稳定可靠，可用于槟榔多酚提取物的定量分析，为槟榔多酚提取物的质量控制提供实验依据。 |
| 中文关键词:槟榔总多酚 儿茶素类 高效液相色谱法 紫外分光光度法 |
| |
| Determination of total polyphenols and catechins in betel nut polyphenol extract |
|
|
| Abstract:Objective To establish a method for the determination of total polyphenols and catechins in betel nut polyphenols extract, and provide reference for the quality control of betel nut polyphenols extract. Methods The content of total phenol in betel nut extract was determined by ultraviolet spectrophotometry. The content of catechins was determined by HPLC. Results The linear range of total polyphenols in betel nuts extract was 9.8~58.8 μg/ml. The three components of catechin, epicatechin and protocatechuic acid were completely separated by HPLC, and the linear relationship was good in their respective ranges, with the recoveries between 99.17% and 101.67%, the RSD between 1.2% and 2.5%. Conclusion The established method is simple, stable and reliable, which could be used for the quantitative analysis of betel polyphenol extract, and provide experimental basis for the quality control of betel polyphenol extract. |
| keywords:betelnut total polyphenols catechins HPLC UV spectrophotometry |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
