| 双波长高效液相色谱法同时测定枇芩口服液中7种成分的含量 |
| 投稿时间：2020-08-03 修订日期：2022-01-06 点此下载全文 |
| 引用本文：王庆芬,刘晓玲,杨育儒,李杰.双波长高效液相色谱法同时测定枇芩口服液中7种成分的含量[J].药学实践杂志,2022,40(2):161~164 |
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| 中文摘要:目的 建立高效液相色谱法同时测定枇芩口服液中绿原酸、咖啡酸、芍药苷、甘草酸铵、黄芩苷、黄芩素和汉黄芩素7种成分含量。方法 采用双波长HPLC，色谱柱为Agilent Zorbax SB-C18(250 mm×4.6 mm，5 μm)；流动相为0.02%磷酸水溶液(A)–乙腈(B)，梯度洗脱。流速：1.0 ml/min；柱温：35 ℃；进样量：20 μl。检测波长：0～18.0 min，325 nm（检测绿原酸、咖啡酸）；18.0～65.0 min，280 nm（检测芍药苷，黄芩苷、黄芩素、甘草酸铵、汉黄芩素）。结果 枇芩口服液中的绿原酸、咖啡酸、芍药苷、甘草酸铵、黄芩苷、黄芩素和汉黄芩素完全分离，7种成分峰面积与浓度的线性关系良好，加样回收率为96.41%～99.70%。结论 该法操作简单、准确、重复性好，可用于枇芩口服液的质量控制。 |
| 中文关键词:枇芩口服液 高效液相色谱 双波长 含量测定 |
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| Simultaneous determination of seven components in Piqin oral liquid by dual-wavelength HPLC |
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| Abstract:Objective To establish a high performance liquid chromatography(HPLC) method for simultaneous determination of the contents of 7 components of chlorogenic acid, caffeic acid, paeoniflorin, ammonium glycyrrhizinate, baicalin, baicalein, and wogonin in Piqin oral liquid. Methods A double-wavelength HPLC method was performed. The column was Agilent Zorbax SB-C18 (250 mm×4.6 mm, 5 μm). The mobile phase was 0.02% phosphoric acid aqueous solution (A)-acetonitrile (B) gradient elution; Flow rate: 1.0 ml/min; Column temperature: 35℃; Injection volume: 20 μl; Detection wavelength: 0-18.0 min, 325 nm (detect chlorogenic acid, caffeic acid); 18.0-65.0 min, 280 nm (detect paeoniflorin, baicalin, baicalein, ammonium glycyrrhizinate, wogonin). Results The chlorogenic acid, caffeic acid, paeoniflorin, ammonium glycyrrhizinate, baicalin, baicalein and wogonin were completely separated. Seven components have a good linear relationship between the peak area and concentration, with the recoveries between 96.41% and 99.70%. Conclusion This method is simple, accurate and reproducible, and can be used for the quality control of Piqin oral liquid. |
| keywords:Piqin oral liquid HPLC double-wavelength content determination |
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