| 复方金钱草颗粒化学成分的UHPLC-Q-TOF/MS分析 |
| 投稿时间：2021-05-26 修订日期：2021-10-28 点此下载全文 |
| 引用本文：张哲,孙卓然,潘鹏超,陈啸飞,柴逸峰.复方金钱草颗粒化学成分的UHPLC-Q-TOF/MS分析[J].药学实践杂志,2022,40(2):146~151,156 |
| 摘要点击次数: 1098 |
| 全文下载次数: 1000 |
|
|
| 中文摘要:目的 通过超高效液相-四极杆飞行时间质谱(UHPLC-Q-TOF/MS)联用技术定性分析中成药复方金钱草颗粒中的主要化学成分。方法 色谱分离采用XBridge BEH C18柱(2.1 mm×100 mm, 2.5 μm)，流动相由0.1%甲酸水和0.1%甲酸乙腈组成，梯度洗脱，流速为0.4 ml/min；质谱定性采用四极杆飞行时间质谱，正离子模式扫描。结果 在优化的液质联用条件下，鉴定出复方金钱草颗粒中47个成分,并通过软件计算区分了其中的同分异构体, 且对其药材来源进行了归属。结论 本研究通过UHPLC-Q-TOF/MS联用技术，为鉴定中成药复方金钱草颗粒中的化学成分建立了一种快速、高效的分析方法。 |
| 中文关键词:复方金钱草颗粒 超高效液相-四极杆飞行时间质谱 化学成分 |
| |
| UHPLC-Q-TOF/MS analysis of chemical constituents in compound Jinqiancao granules |
|
|
| Abstract:Objective To qualitatively analyze the main chemical components in compound Jinqiancao granules by ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Methods XBridge BEH C18 column (2.1 mm×100 mm, 2.5 μm) was used for chromatographic separation. The mobile phase was composed of 0.1% formic acid water and 0.1% formic acid-acetonitrile, gradient elution, and the flow rate was 0.4 ml/min. Mass spectrometry was characterized by Quadrupole time-of-flight mass spectrometry (Q-TOF/MS) and positive ion mode scanning. Results Under the optimized LC/MS condition, 47 components in compound Jinqiancao granules were identified. The isomers were distinguished by software calculation. The source of medicinal materials was assigned. Conclusion A rapid and efficient analytical method was established for the identification of chemical components in compound Jinqiancao granules by UHPLC-Q-TOF/MS. |
| keywords:compound Jinqiancao granules UHPLC-Q-TOF/MS chemical constituents |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
