| CRELD2在小鼠组织中的表达水平研究 |
| 投稿时间：2021-11-09 修订日期：2022-03-12 点此下载全文 |
| 引用本文：常富强,王淑娜,汪东昇,缪朝玉.CRELD2在小鼠组织中的表达水平研究[J].药学实践杂志,2022,40(2):125~131 |
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| 基金项目:国家自然科学基金重点项目(No 82030110); 上海市2021年度“科技创新行动计划”实验动物研究领域项目(No 21140901000) |
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| 中文摘要:目的 通过不同方法在基因及蛋白质水平探究CRELD2在小鼠各组织中的表达水平，为研究CRELD2在各组织中的生物学功能提供依据。方法 通过实时荧光定量聚合酶链式反应（RT-PCR）及蛋白质印迹法（Western Blot，WB）测定C57BL/6J小鼠的肝、胰腺、胃、肺等组织中CRELD2 mRNA与CRELD2蛋白的含量，实现在不同水平上探究CRELD2在各组织中的表达情况。结果 RT-PCR及WB测定结果显示，CRELD2在小鼠的肝、胰腺、胃、肺组织中均有表达，表达量在各组织间存在差异；基因水平的相对表达量排序为：胰腺>胃>肝>肺；蛋白水平的相对表达量排序为：胰腺>肝>胃>肺。检测结果表明CRELD2在各组织中普遍存在，但组织间相对表达量的排序并不完全一致，推测与转录调控相关。结论 CRELD2在小鼠的肝、胰腺、胃、肺组织中均有表达，且相对表达量在基因与蛋白质水平不完全平行。 |
| 中文关键词:CRELD2 组织特异性 分泌蛋白 |
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| Expression of CRELD2 in mouse tissues |
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| Abstract:Objective To explore the expression of CRELD2 at the gene and protein levels of mouse tissues, and to provide a reference for studying the biological function of CRELD2 in various tissues. Methods The expression level of CRELD2 in the liver, pancreas, stomach, and lung of C57BL/6J mice was determined by real-time PCR and Western Blot. Results RT-PCR and WB showed that CRELD2 was expressed in mouse liver, pancreas, stomach, and lung. The relative expression levels of CRELD2 from high to low were pancreas, stomach, liver, and lung at the gene level, and pancreas, liver, stomach, and lung at protein level respectively. The result suggested that the relative expression levels of the CRELD2 gene and protein in different tissues were not completely consistent, suggesting that it is related to transcriptional regulation. Conclusion CRELD2 is expressed in mouse liver, pancreas, stomach, and lung, and the relative expression levels of CRELD2 are not completely parallel at the gene and protein level. |
| keywords:CRELD2 tissue specificity secreted protein. |
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