| 基于网络药理学的益母草治疗神经损伤的潜在分子机制研究 |
| 投稿时间：2021-05-22 修订日期：2021-10-26 点此下载全文 |
| 引用本文：张倩,郭羽晨,邓珊珊,章越凡,李铁军.基于网络药理学的益母草治疗神经损伤的潜在分子机制研究[J].药学实践杂志,2022,40(2):113~119 |
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| 基金项目:上海市浦东新区卫生系统新兴、交叉学科（精准临床药学，PWXx2020-03） |
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| 中文摘要:目的 探讨益母草治疗神经损伤的作用机制。方法 检索中药系统药理学数据库（TCMSP）和中药分子机制的生物信息学数据库（BATMAN-TCM）获取益母草的活性成分，然后从SwissTargetPrediction (STP)和TCMSP数据库中收集和预测候选化合物的作用靶点。利用疾病靶点标准化数据库（Uniprot）获取益母草活性成分对应的靶标基因，与Genecards、DisGenet、OMIM三个数据库获取的神经损伤的疾病基因做映射，获得益母草治疗神经损伤的潜在作用靶点。其次借助网络拓扑分析软件Cytoscape 3.6.0构建益母草活性成分-作用靶点网络。通过蛋白互作平台数据库（STRING）构建作用靶点之间的互作关系（PPI），将其导入Cytoscape3.6.0软件构建PPI网络图。最后，通过STRING数据库对益母草治疗神经损伤的作用靶点进行GO富集分析和KEGG通路富集分析。结果 从益母草中筛选出19个有效活性成分，涉及654个作用靶点，其中与神经损伤有关的作用靶点426个，关键靶点6个，这些靶点基因主要参与生物调节、氧化应激反应、细胞通讯等生物学过程。分子功能主要与蛋白质结合、离子结合和催化还原有关，它们在细胞膜外侧富集。其机制与MAPK、Toll样受体、PI3K-Akt、肿瘤坏死因子、IL-17和细胞凋亡等信号通路等有关。结论 益母草的活性成分可能是通过抗炎、抗凋亡和促进细胞生长来发挥神经损伤的保护作用。 |
| 中文关键词:益母草 网络药理学 神经损伤 |
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| Potential molecular mechanism of motherwort in the treatment of nerve injury based on network pharmacology |
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| Abstract:Objective To explore the mechanism of motherwort in the treatment of nerve injury. Methods The active components of motherwort were obtained by searching TCMSP and BATMAN-TCM databases. The action targets of candidate compounds were collected and predicted from TCMSP and SwissTargetPrediction (STP) databases. The target genes corresponding to the active components of motherwort were obtained by using the standardized database of disease targets (Uniprot). The potential targets of motherwort in the treatment of nerve injury were obtained by mapping the disease genes of nerve injury with the three databases of Genecards, DisGenet and OMIM. The network topology analysis software Cytoscape 3.6.0 was used to construct the action target network of motherwort active components. The protein interaction platform database (STRING) was used to construct the interaction relationship between action targets. The target protein interaction (PPI) network was constructed by introducing Cytoscape 3.6.0 software. Through STRING database, GO enrichment analysis and KEGG pathway enrichment analysis were carried out to analyze the target points of motherwort in the treatment of nerve injury. Results 19 active components were screened from motherwort, involving 654 action targets, including 426 action targets related to nerve injury and 6 key targets. These target genes were mainly involved in biological regulation, oxidative stress response and cell communication and other biological processes. Molecular functions were mainly related to protein binding, ion binding and catalytic reduction. They were enriched outside the cell membrane. Its mechanism was related to signal pathways such as MAPK, Toll-like receptor, PI3K-Akt, TNF, IL-17, and apoptosis. Conclusion The active components of motherwort may play a protective role on nerve injury through anti-inflammation, anti-apoptosis and promoting cell growth. |
| keywords:motherwort network pharmacology nerve injury |
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