| 泰山白首乌内生真菌多样性及其抗肿瘤活性研究 |
| 投稿时间：2021-08-18 修订日期：2021-10-25 点此下载全文 |
| 引用本文：冯坤苗,吴思佳,陈文华,代威,徐凌川,韩婷.泰山白首乌内生真菌多样性及其抗肿瘤活性研究[J].药学实践杂志,2021,39(6):542~548 |
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| 中文摘要:目的 分析白首乌内生真菌多样性及其种群结构分布规律，挖掘潜在的微生物资源及功能，为寻找新的抗肿瘤内生真菌提供理论基础。方法 通过传统的内生菌分离法以及18sRNA高通量测序技术，对不同组织部位、不同物种和不同产地白首乌内生真菌群落组成进行多样性分析；采用 MTT 法检测泰山白首乌内生真菌的细胞毒活性。结果 从泰山白首乌的根、茎、叶中共分离得到 90个形态的内生真菌，其中，镰刀菌属Fusarium和链格孢属Alternaria 为优势菌属;泰山白首乌根、茎、叶分别有8、9和13个属，链格孢属Alternaria和炭疽菌属Colletotrichum为共有属; 13株泰山白首乌内生真菌对HEPG2、HGC27、HT-29和HeLa肿瘤细胞株产生抗肿瘤活性，占总数的14.4%,极细链格孢菌A. tenuissima LTJ2和链格孢菌A. alternata LTJ6抗肿瘤活性显著。结论 泰山白首乌内生真菌具有丰富的多样性，部分菌株具有显著的抗肿瘤活性，为寻找新的抗肿瘤药物提供了菌株资源。 |
| 中文关键词:泰山白首乌 链格孢菌 抗肿瘤活性 |
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| Anti-tumor activities of endophytes from Cynanchum bungei Decne. |
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| Abstract:Objective To investigate the taxonomic structure and diversity of endophytic fungi from Cynanchum bungei Decne., explore the potential microbial resources and functions and provide the theoretical basis for new antitumor endophytic fungi. Methods The diversities of endophytic fungi community in different tissues, species and habitats were analyzed with traditional endophytic bacteria separation method and 18sRNA high-throughput sequencing technology. MTT assay was used to detect the cytotoxic activity of endophytic fungi from Radix Polygoni multiflori. Results 90 strains of endophytic fungi were isolated and identified from roots, stems, and leaves of C. bungei. Among them, Fusarium and Alternaria were the dominant genera. There were 8, 9 and 13 genera from roots, stems and leaves of C. bungei respectively. Among which Alternaria and Colletotrichum were the common genera in different tissues. Further studies showed that 13 endophytic fungi of C.bungei had good anti-tumor activity in vitro, accounting for 14.4% of the total genera. Among them, A. tenuissima LTJ2 and A. alternata LTJ6 had significant anti-tumor activity. Conclusion The endophytic fungi in Cynanchum bungei Decne. have rich diversity. Some strains have significant anti-tumor activity，which can be potential resources for the development of new antitumor agents. |
| keywords:C. bungei Alternaria anti-tumor activity |
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