| 不同途径注射肿瘤RNA纳米脂质体疫苗对结肠癌生长的影响 |
| 投稿时间：2021-08-19 修订日期：2021-10-25 点此下载全文 |
| 引用本文：卢光照,张翮,樊莉,孙治国,鲁莹.不同途径注射肿瘤RNA纳米脂质体疫苗对结肠癌生长的影响[J].药学实践杂志,2021,39(6):520~524,556 |
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| 基金项目:国家自然科学基金（81773278，30801441） |
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| 中文摘要:目的 本研究旨在比较CT26肿瘤细胞RNA负载于纳米脂质体后，使用不同途径注射所引起的生物体抗肿瘤生长的作用差异。方法 将提取的肿瘤RNA载入到纳米脂质体制备成肿瘤RNA纳米脂质体疫苗，然后进行脂质体疫苗表征。肿瘤RNA纳米脂质体疫苗粒径为（120.0±12.1）nm，电位为（3.39±0.56）mV。最后在小鼠不同部位注射肿瘤RNA纳米脂质体疫苗，检测分析不同部位注射对小鼠结肠癌移植瘤生长的影响。结果 通过肿瘤RNA纳米脂质体疫苗对移植瘤小鼠进行不同途径注射治疗，结果显示，相比皮下注射，腹腔注射能够更加有效地增强生物体抗肿瘤免疫反应，抑制移植瘤生长。最后，比较小鼠重要脏器的H&E染色，均未见脏器有明显的器质性病变。结论 通过腹腔注射肿瘤RNA纳米脂质体疫苗比皮下注射能够更加有效地增强机体抗肿瘤免疫反应。 |
| 中文关键词:CT26结肠癌细胞 纳米脂质体 疫苗 肿瘤RNA |
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| Effect of different injection approaches of tumor RNA nanoliposome vaccine on the growth of colon cancer |
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| Abstract:Objective To compare the differences in the anti-tumor growth effects of organisms with different injections of CT26 tumor cell RNA loaded into nanoliposomes. Methods The extracted tumor RNA was loaded into nanoliposomes to prepare tumor RNA nanoliposome vaccines, and the related properties of nanoliposome vaccines were investigated. The particle size of nanoliposome vaccines was （120.0±12.1）nm and zeta potential was （3.39±0.56）mV. Tumor RNA nanoliposome vaccines were injected into different parts of the mice to test and analyze the influence of different injections on the growth of colon cancer transplanted tumors in mice. Results Tumor RNA nanoliposome vaccines were used to inject tumor-transplanted mice in different ways. Compared with underarm injection, intraperitoneal injection enhanced the organism's anti-tumor immune response and inhibited the growth of transplanted tumors more effectively. The H&E staining of important organs in mice was compared and no obvious organic lesions were found in the organs. Conclusion Intraperitoneal injections of nanoliposome loaded with tumor RNA can enhance the body's anti-tumor immune response more effectively than underarm injections. |
| keywords:CT26 colon cancer cells nanoliposomes vaccine tumor RNA |
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