| 血红铆钉菇多糖对RAW264.7巨噬细胞免疫调节作用研究 |
| 投稿时间：2021-02-01 修订日期：2021-08-22 点此下载全文 |
| 引用本文：豆甲泰,刘友昊,梁启超,吴宜艳.血红铆钉菇多糖对RAW264.7巨噬细胞免疫调节作用研究[J].药学实践杂志,2021,39(5):449~453 |
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| 中文摘要:目的 研究血红铆钉菇多糖对RAW264.7小鼠单核巨噬细胞的免疫调节作用。方法 将小鼠的巨噬细胞进行复苏培养，制备细胞悬液，设置空白对照组以及不同质量浓度的血红铆钉菇多糖（CRPS25-Ⅱ）组（1、20、40、80和160 μg/ml）。采用MTT法测定血红铆钉多糖对小鼠巨噬细胞RAW264.7的细胞毒性；采用RT-PCR法检测血红铆钉菇多糖对小鼠巨噬细胞RAW264.7分泌免疫调节因子IL-6和TNF-α的影响；采用Western-blot法检测血红铆钉菇多糖对小鼠巨噬细胞RAW264.7的NF-κB信号通路中p-P65蛋白表达的影响。结果 实验证明血红铆钉菇多糖在1～160 μg/ml的范围内没有明显的细胞毒性，CRPS25-Ⅱ质量浓度在1～160 μg/ml可提高细胞因子的分泌量，从而促进细胞因子IL-6和TNF-α的mRNA表达；CRPS25-Ⅱ可促进p-P65蛋白的磷酸化，激活NF-κB信号通路从而促进细胞的免疫调节作用，1～160 μg/ml浓度范围内均可促进p-P65蛋白的增加，1～40 μg/ml呈上升趋势，40～160 μg/ml促进作用逐渐减弱。结论 血红铆钉菇多糖对小鼠巨噬细胞无毒性，且可以促进炎症因子IL-6和TNF-α的分泌以及激活NF-κB信号通路，从而起到免疫调节作用。 |
| 中文关键词:血红铆钉菇多糖 巨噬细胞 免疫调节 |
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| Immunoregulatory effect of polysaccharides derived from chroogomphus rutilus on macrophage cell line RAW264.7 |
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| Abstract:Objective To study the immunomodulatory effect of polysaccharides (CRPS25-Ⅱ) derived from Chroogomphus rutilus on mouse mononuclear macrophages, RAW264.7 cells.Methods RAW264.7 cells were resuspended and cultured, cell suspension was prepared. The blank control group and CRPS25-Ⅱ groups with different mass concentrations (1, 20, 40, 80 and 160 μg/ml) were set up. MTT assay was used to determine the cytotoxicity of CRPS25-Ⅱ on RAW264.7 cells. RT-PCR was used to detect the effects of CRPS25-Ⅱ on the secretion of immune regulatory factors IL-6 and TNF-α from RAW264.7 cells. Western blot was used to detect the effects of CRPS25-Ⅱ on the expression of p-P65 protein in NF-κB pathway of RAW264.7 cells.Results The results showed that CRPS25-Ⅱ (1-160 μg/ml) had no obvious cytotoxicity. CRPS25-Ⅱ (1-160 μg/ml) increased the secretion of cytokines, and thus promoted the mRNA expression of IL-6 and TNF-α. CRPS25-Ⅱ increased the phosphorylation of p-P65 protein and activated the NF-κB signaling pathway, and thus promoted the immune regulation of cells. CRPS25-Ⅱ (1-160 μg/ml) could increase the p-P65 protein, and the promoting effects of CRPS25-Ⅱshowed an upward trend in the concentration range of 1-40 μg/ml and gradually weakened in the concentration range of 40-160 μg/ml.Conclusion Polysaccharides derived from chroogomphus rutilus had no cytotoxicity to mouse macrophages, and could promote the secretion of inflammatory factors IL-6 and TNF-α and activate the NF-κB signaling pathway, thus playing an immunomodulatory role. |
| keywords:CRPS25-Ⅱ RAW264.7 cells immune regulation |
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