| 用HPLC法测定红茴香注射液中槲皮苷的含量 |
| 投稿时间：2021-03-19 修订日期：2021-06-30 点此下载全文 |
| 引用本文：成程,陈向梅,马健,赵氚.用HPLC法测定红茴香注射液中槲皮苷的含量[J].药学实践杂志,2021,39(5):447~448,453 |
| 摘要点击次数: 1018 |
| 全文下载次数: 797 |
|
|
| 中文摘要:目的 建立高效液相色谱法测定红茴香注射液中槲皮苷的含量。方法 色谱柱为Dikma C18(250 mm×4.6 mm，5 μm)，以乙腈-0.1%磷酸水溶液（25∶75）为流动相，流速为1.0 ml/min，柱温30 ℃，检测波长为256 nm。结果 槲皮苷在0.215～3.225 μg范围内呈良好线性关系，相关系数为0.999 6，该法的平均回收率为99.39%，RSD为0.82%（n=6），重复性为1.194 mg/ml，RSD为0.40%。结论 3批红茴香注射液的槲皮苷平均含量结果为1.191 mg/ml，该法简便、快速、准确, 可作为测定红茴香注射液中槲皮苷含量的方法。 |
| 中文关键词:高效液相色谱法 红茴香注射液 槲皮苷 |
| |
| Quantitative determination of quercetin in Honghuixiang injection by HPLC |
|
|
| Abstract:Objective To establish quantitative methods to assay quercetin in Honghuixiang injection by HPLC.Methods Dikma C18 column(250 mm×4.6 mm, 5 μm) was used for the assay with acetonitrile -0.1% phosphoric acid (25∶75) as the mobile phase. Flow rate was 1.0 ml/min. The column temperature was 30 ℃. The detection wavelength was at 256 nm.Results Quercetin showed good linear relationship within the range of 0.2150–3.225 μg. The correlation coefficient was 0.999 6. The average recovery was 99.39% with RSD 0.82% (n=6). The repeatability was 1.194 mg/ml with RSD 0.40%.Conclusion The average quercetin content in three batches of Honghuixiang injection was 1.191 mg/ml. This method is simple, rapid and accurate. It can be used for the determination of quercetin in Honghuixiang injection. |
| keywords:HPLC Honghuixiang injection quercetin |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
