| 马尾松叶低极性部位的GC-MS分析及协同氟康唑抗耐药白念珠菌活性研究 |
| 投稿时间：2021-01-05 修订日期：2021-05-15 点此下载全文 |
| 引用本文：孔飞飞,郑巍,郭良君,金永生.马尾松叶低极性部位的GC-MS分析及协同氟康唑抗耐药白念珠菌活性研究[J].药学实践杂志,2021,39(5):399~402,441 |
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| 基金项目:浙江省湖州市公益性技术应用研究（No. 2018GY17） |
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| 中文摘要:目的 研究马尾松叶低极性部位的主要化学成分，以及其与氟康唑联用对氟康唑耐药白念珠菌的抗真菌活性。方法 马尾松叶经80%乙醇热提取后，用石油醚萃取得到低极性部位，运用GC-MS法检测其化学成分，所测结果与标准谱库进行比对分析，用峰面积归一法计算各个成分的相对含量。采用棋盘式微量稀释法测试低极性部位协同氟康唑对耐药白念珠菌的最小抑菌浓度（MIC80）。结果 从低极性部位中检测出30种成分，鉴定了17个化学成分；马尾松叶80%乙醇提取物、低极性部位及石油醚提取物协同氟康唑对耐药白念珠菌的最小抑菌浓度（MIC80）分别为7.81 μg/ml、31.25 μg/ml、>250 μg/ml。结论 马尾松叶80%乙醇提取物及低极性部位具有协同氟康唑抗耐药白念珠菌活性；马尾松叶低极性部位协同氟康唑抗耐药白念珠菌活性的有效成分可能是其中的二萜类化合物（53.99%）。 |
| 中文关键词:马尾松 低极性部位 气质联用 耐药白念珠菌 氟康唑 |
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| GC-MS analysis of low polarity extracts from Pinusmassoniana Lamb. leaves and study on their synergetic activity of fluconazole against fluconazole-resistant Candida albicans |
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| Abstract:Objective To investigate the main chemical constituents of the low polarity extracts from pinusmassoniana Lamb. leaves and their synergetic activity with fluconazole against fluconazole-resistant Candida albicans.Methods The pinusmassoniana leaves were extracted with 80% ethanol, and then the extracts were extracted by petroleum ether to obtain the low polarity extracts. The chemical components were detected by GC-MS and elucidated by the comparison with the standard mass spectral data. The relative contents in percentage were calculated using the area normalization method. The minimal inhibitory concentrations (MIC80) of fluconazole-resistant Candida albicans strains by the low polarity extracts in combination with fluconazole were determined by checkerboard microdilution assay.Results 30 components were detected from the low polarity extracts, and 17 components were identified. The minimum inhibitory concentration (MIC80) of the 80% ethanol extracts, the low polarity extracts and the petroleum ether extracts from the pinusmassoniana leaves combined with fluconazole against fluconazole-resistant Candida albicans were 7.81 μg/ml, 31.25 μg/ml and >250 μg/ml, respectively.Conclusion The 80% ethanol extracts of pinusmassoniana leaves and its low polarity extracts have synergistic activity combined with fluconazole onfluconazole-resistant Candida albicans. The diterpenoids (53.99%) may be the effective components of the low polarity extracts. |
| keywords:Pinusmassoniana Lamb. low polarity extracts GC-MS fluconazole-resistant Candida albicans fluconazole |
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