| 赶黄草中大环多酚类成分含量测定及其提取工艺 |
| 投稿时间：2020-09-14 修订日期：2021-01-11 点此下载全文 |
| 引用本文：陈岚,江圣圭,史鹏杰,蔡舒心,董志颖,孙连娜.赶黄草中大环多酚类成分含量测定及其提取工艺[J].药学实践杂志,2021,39(4):309~312,378 |
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| 基金项目:国家重点研发计划资助（2019YFC1711000）；上海市自然科学基金项目（13401900106） |
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| 中文摘要:目的 建立HPLC法同时测定赶黄草中大环多酚类成分：乔松素-7-O-[4'', 6''-(S)-六羟基二苯甲酰基]-β-D-葡萄糖苷（PHG）、乔松素-7-O-[3''-O-没食子酰基-4'', 6''-(S)-六羟基二苯甲酰基]-β-D-葡萄糖苷（PGHG）、乔松素二氢查耳酮-7-O-[3''-O-没食子酰基-4'', 6''-(S)-六羟基二苯甲酰基]-β-D-葡萄糖苷（THA）的含量，并优化其最佳提取工艺。方法 通过HPLC法测定PHG、PGHG、THA的含量，以PHG、PGHG、THA的总提取率为指标对赶黄草提取物进行分析，采用正交设计考察溶媒浓度、提取时间、溶媒用量、提取次数对提取率的影响，从而优化赶黄草大环多酚类成分的最佳提取工艺。结果 在采用的含量测定方法下，PHG、PGHG、THA在线性范围内线性关系良好，加样回收率在100.90%～102.04%之间，RSD值均小于1.5%。最佳提取工艺为取赶黄草干药材，切3～5 cm小段，加入10倍体积、浓度为80%的乙醇水溶液，回流2次，每次2 h。该工艺下，大环多酚提取率超过90%。结论 新建立的含量测定方法准确稳定，重复性好；经优化的提取工艺稳定可行，可为该类成分的进一步开发利用打下基础。 |
| 中文关键词:赶黄草 高效液相色谱 PHG PGHG THA 正交设计 提取工艺 |
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| Content determination and extraction process of macrocyclic polyphenols from Penthorum chinense Pursh |
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| Abstract:Objective To establish an HPLC method for simultaneous assay of macrocyclic polyphenols from Penthorum chinense Pursh, pinocembrin-7-O-[4'', 6''-(S)-hexahydroxydiphenoyl]-β-D-glucose (PHG), pinocembrin-7-O-[3''-O-galloyl-4'', 6''-(S)-hexahydroxydiphenoyl]-β-D-glucose (PGHG) and pinocembrin dihydrochalcone-7-O-[3''-O-galloyl-4'', 6''-(S)-hexahydroxydiphenoyl]-β-D-glucoside or thonningianin A (THA), and optimize the extraction process.Methods The total extraction rate of PHG, PGHG, THA was used as an investigated index to analyze the extracts from Penthorum chinense Pursh. Orthogonal design was applied to evaluate solvent amount, extraction time, solvent concentration and extraction times as the influencing factors for the optimal extraction process of macrocyclic polyphenols from Penthorum chinense Pursh.Results When this content assay method was adopted, there were good linear relationships for PHG, PGHG, THA in the linear range. The recoveries were between 100.90% to 102.04% with the RSDs below 1.5%. The optimal extraction process was involved in cutting Penthorum chinense Pursh into 3-5 cm, adding 10 times 80% ethanol aqueous solution by volume and refluxing 2 hours twice. The extraction rate of macrocyclic polyphenols was above 90% with this process.Conclusion This assay method is accurate, stable, and repeatable. The optimized extraction process is stable and feasible for further development and utilization. |
| keywords:Penthorum chinense Pursh HPLC PHG PGHG THA orthogonal method extraction process |
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