| 用HPLC法同时测定养血安神糖浆中4种成分的含量 |
| 投稿时间：2020-06-18 修订日期：2020-10-21 点此下载全文 |
| 引用本文：陆萍,倪东杰,郑巍,郭良君,王翔.用HPLC法同时测定养血安神糖浆中4种成分的含量[J].药学实践杂志,2021,39(3):270~273 |
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| 中文摘要:目的 建立同时测定养血安神糖浆中槲皮苷、木犀草苷、芦丁和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的方法。方法 采用HPLC法。色谱柱为Waters Symmetry C18（4.6 mm×250 mm，5 μm）。流动相：0.1%醋酸（A）-甲醇（B）梯度洗脱，0～15 min，95%～90%A；15～35 min，90%～70%A；35～55 min，70%～60%A；55～85 min，60%～50%A；85～95 min，10%A。流速：1.0 ml/min，检测波长：256 nm和320 nm，柱温30 ℃，进样量10 μl。结果 槲皮苷、木犀草苷、芦丁和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷分别在10～300、5.0～150.0、5.0～150.0、20.0～600.0 μg/ml范围呈良好的线性关系（r≥0.9989）；加样回收率分别为（96.75±1.41）%、（99.61±1.01）%、（97.18±1.96）%和（99.12±0.97）%（n = 6）。结论 该方法简单、准确、稳定，可同时测定养血安神糖浆中4种成分的含量。 |
| 中文关键词:养血安神糖浆 槲皮苷 木犀草苷 芦丁 2,3,5,4’-四羟基-二苯乙烯-2-O-β-D-葡萄糖苷 高效液相色谱法 |
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| One step assay of the four plant ingredients in Yangxue Anshen syrup by HPLC method |
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| Abstract:Objective To establish a HPLC method for simultaneous determination of quercitrin, luteoloside, rutin and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Yangxue Anshen syrup.Methods Waters symmetry C18 column (250 mm×4.6 mm, 5 μm) was used with 0.1% acetic acid (A) and methanol (B) as the mobile phase. Gradient elution was performed at a flow rate of 1.0 ml/min, 0-15 min, 95%-90%A; 15-35 min, 90%-70%A; 35-55 min, 70%-60%A; 55-85 min, 60%-50%A; 85-95 min, 10%A. The detection wavelengths were 256 nm and 320 nm. Column temperature was 30 ℃ and the injection volume was 10 μl.Results Quercitrin, luteoloside, rutin and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside showed good linear relationship within the range of 10-300, 5.0-150.0, 5.0-150.0, 20.0-600.0 μg/ml(r≥0.9989), respectively. The average recovery was (96.75±1.41)%, (99.61±1.01)%, (97.18±1.96)% and(99.12±0.97)% (n=6), respectively.Conclusion The established method is simple, accurate and stable, which can be used for the simultaneous determination of 4 components in Yangxue Anshen syrup. |
| keywords:Yangxue Anshen syrup quercitrin luteoloside rutin 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside HPLC |
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