| 啤酒花及其活性成分黄腐酚抗糖皮质激素性骨质疏松的作用研究 |
| 投稿时间：2021-02-02 修订日期：2021-04-22 点此下载全文 |
| 引用本文：夏天爽,丁卢颖,张嘉宝,李晓瑾,王果平,辛海量.啤酒花及其活性成分黄腐酚抗糖皮质激素性骨质疏松的作用研究[J].药学实践杂志,2021,39(3):221~225 |
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| 基金项目:国家自然科学基金资助项目（U1603283，82004015） |
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| 中文摘要:目的 研究对啤酒花及其活性成分黄腐酚抗糖皮质激素性骨质疏松（GIOP）作用。方法 腹腔注射地塞米松（DEX）造模，并结合Micro-CT及Elisa试剂盒检测等方法，对小鼠股骨的骨微结构、骨密度及血清骨生化指标进行评价。同时，采用DEX损伤成骨细胞，对其增殖、分化水平及骨形成相关蛋白的表达进行评价。结果 啤酒花提取物及黄腐酚可显著改善GIOP小鼠的骨微结构破坏，增强骨密度，改善骨小梁参数。在成骨细胞水平上，啤酒花及黄腐酚既可促进DEX损伤成骨细胞的增殖、分化，又可提高骨钙素（BGP）、骨形成蛋白2（BMP-2）以及成骨特异性转录因子（Runx-2）的表达水平，促进骨形成。结论 该研究首次明确了啤酒花及黄腐酚具有抗GIOP作用，为抗骨质疏松药物的开发提供了新资源。 |
| 中文关键词:啤酒花 黄腐酚 糖皮质激素性骨质疏松 地塞米松 成骨细胞 |
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| Effects of Humulus lupulus L. and its active ingredient xanthohumol on preventing glucocorticoid-induced osteoporosis |
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| Abstract:Objective To explore the effects of Humulus lupulus L. extract (HLE) and xanthohumol (XN) on preventing glucocorticoid-induced osteoporosis (GIOP).Methods The GIOP model was established by intraperitoneal injection of dexamethasone (DEX). Bone microstructure, bone mineral density and serum biochemical indexes were evaluated by Micro-CT and ELISA kits. The levels of cells proliferation and ALP activity, and the expression of bone formation related proteins were assayed with primary osteoblasts injured by DEX.Results HLE and XN significantly alleviated the bone microstructure damage, enhanced the bone mineral density, and improved the trabecular parameters in GIOP mice. In vitro experiments showed that HLE and XN can prevent bone loss not only by improving cell proliferation and ALP activity, but also through increasing the expression of bone γ-glutamic acid-containing proteins (BGP), bone morphogenetic protein 2 (BMP-2) and runt-related transcription factor 2 (Runx-2).Conclusion This study confirmed that HLE and XN had anti-GIOP effects for the first time. It provides a new resource for the development of anti-osteoporosis medications. |
| keywords:Humulus lupulus L. xanthohumol glucocorticoid-induced osteoporosis dexamethasone osteoblast |
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