| UHPLC-MS/MS法测定新冠肺炎经验方寒湿郁肺汤中11种主成分的含量 |
| 投稿时间：2020-12-05 修订日期：2021-03-13 点此下载全文 |
| 引用本文：崔莉莉,陈勇,张蒙伟,鄢红霞,高守红,陈万生.UHPLC-MS/MS法测定新冠肺炎经验方寒湿郁肺汤中11种主成分的含量[J].药学实践杂志,2021,39(2):148~151,156 |
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| 基金项目:上海市临床药学重点专科建设项目（2016-40044-002）；上海市卫生计生系统重要薄弱学科建设项目（2016ZB0303） |
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| 中文摘要:目的 建立测定寒湿郁肺汤中11个主成分含量的方法。方法 采用超高效液相色谱串联质谱法（UHPLC-MS/MS），色谱柱为Agilent ZORBAX SB-C18（3.5 μm，2.1 mm×150 mm ），流动相为0.2%甲酸-10 mmol/L醋酸铵水溶液（A）-乙腈（B），梯度洗脱(0～0.6 min, 80%～40%A; 0.6～1 min, 40%～30%A; 1～4.3 min, 30%～5%A)，流速0.3 ml/min，柱温40 ℃。以利血平为检测内标，采用多反应监测模式同时测定香草酸、厚朴酚、和厚朴酚、汉黄芩素、刺槐素、6-姜酚、桔皮素、羌活醇、川陈皮素、紫花前胡苷、橙皮苷11个化学主成分的含量。结果 寒湿郁肺汤中11个主成分在各自浓度范围内线性关系良好（r>0.98），平均回收率在93.11%～111.73%。结论 该实验所建立的方法操作简便、重复性好，为寒湿郁肺汤的质量控制提供了实验室依据。 |
| 中文关键词:超高效液相色谱串联质谱法 含量测定 新型冠状病毒肺炎 寒湿郁肺汤 |
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| Determination of 11 main components in Hanshi Yufei decoction, a COVID-19 empirical prescription by UHPLC-MS/MS |
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| Abstract:Objective To establish a method to determine 11 main components in Hanshi yufei decoction.Methods The method adopted UHPLC-MS/MS with an Agilent ZORBAX SB-C18 (3.5 μm，2.1 mm×150 mm) column. The mobile phase was consisted of 0.2% formic acid plus 10 mmol/L ammonium acetate aqueous solution(A) - acetonitrile(B) and gradient elution (0–0.6 min, 80%–40%A; 0.6–1 min, 40%–30%A; 1–4.3 min, 30%–5%A) at 0.3 ml/min. The column temperature was 40 ℃ and 11 main components including vanillic acid, magnolol, honokiol, wogonin, sophorin, 6-gingerol, citrinin, qianghuo alcohol, nobiletin, nodakenin, and hesperidin were quantified in a multiple reaction monitoring mode. The reserpine was the standard.Results The 11 main components in Hanshi Yufei decoction had a good linear relationship within their concentration range (r>0.98), and the average recovery was 93.11%～111.73%.Conclusion The UHPLC-MS/MS method established in this experiment is easy to operate and has good reproducibility, which provides a laboratory basis for the quality control of Hanshi Yufei decoction. |
| keywords:UHPLC-MS/MS content determination COVID-19 Hanshi Yufei decoction |
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