| 微波消解-电感耦合等离子体原子发射光谱法测定全血中的镧 |
| 投稿时间：2020-12-10 修订日期：2020-12-07 点此下载全文 |
| 引用本文：张静如,华俊杰,韩凌,朱敏,鲁莹.微波消解-电感耦合等离子体原子发射光谱法测定全血中的镧[J].药学实践杂志,2021,39(2):118~120,125 |
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| 基金项目:国家科技重大专项(子课题)－长远航环境及应激损伤防治药物的临床前及临床研究（2018ZX09J18110-001） |
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| 中文摘要:目的 评估多效防护乳中镧（La）的透皮安全性，建立微波消解-电感耦合等离子体发射光谱（ICP-OES）法测定大鼠全血中镧的分析方法。方法 全血样品经微波消解后，采用电感耦合等离子发射光谱仪分析，以333.749 nm为分析谱线，对大鼠全血中的La进行含量测定。结果 该方法的标准曲线相关性良好（r>0.9994），方法检出限为0.0025 μg/ml，定量限为0.0077 μg/ml，精密度<3%，回收率在94.9%～102.0%之间。结论 基于微波消解的ICP-OES方法稳定可靠，可为镧元素的含量测定和给药安全性研究提供重要依据。 |
| 中文关键词:镧 微波消解 多效防护乳 电感等离子体原子发射光谱 透皮 |
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| Determination of lanthanum in whole blood by microwave digestion-inductively coupled plasma optical emission spectrometry |
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| Abstract:Objective To evaluate the transdermal safety of lanthanum (La) in sunscreen and jellyfish sting protective lotion, establish a microwave digestion-inductively coupled plasma opticalemission spectroscopy (ICP-OES) method for determination of lanthanum (La) in rat’s whole blood.Methods The whole blood samples were digested by microwave and analyzed by inductively coupled plasma emission spectrometer (ICP-OES). Using 333.749 nm as the analysis line, the content of La in rat whole blood was determined.Results The correlation linearity of the standard curve of this method was good (r>0.9994), the detection limit of the method was 0.0025 μg/ml, the limit of quantification was 0.0077 μg/ml, the precision was less than 3%, and the recovery rate was between 94.9% and 102.0%.Conclusion The ICP-OES method based on microwave digestion is stable and reliable, and can provide an important basis for the study of the transdermal safety of lanthanum. |
| keywords:lanthanum microwave digestion sunscreen and jellyfish sting protective lotion inductive plasma optical emission spectrometry transdermal |
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