| 不同鸟嘌呤碱基数目对DNA/银纳米簇荧光强度的影响 |
| 投稿时间：2020-10-13 修订日期：2021-03-06 点此下载全文 |
| 引用本文：袁一凡,陆峰.不同鸟嘌呤碱基数目对DNA/银纳米簇荧光强度的影响[J].药学实践杂志,2021,39(2):108~111 |
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| 基金项目:国家科技重大专项（2018ZX09J18112）、国家重点研发计划（2019YFC0312603）、军队生物安全研究专项（19SWAQ20-04） |
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| 中文摘要:目的 通过C-G碱基互补配对的方式，考察不同鸟嘌呤碱基(G)数目对DNA/银纳米簇荧光信号强度的影响，以此来探究新的荧光探针开关构建的方法。方法 利用核酸碱基互补配对原则，设计了一系列银纳米簇的适配体部分的互补序列,考察了银纳米簇的适配体序列中暴露的G碱基个数对荧光信号的影响。结果 碱基G可增强银纳米簇的荧光信号强度，且荧光信号强度与G碱基个数呈现正相关关系，拟合线性方程为Y=1726.1X+8972.5，r=0.9789。结论 该实验研究对于调节银纳米簇的荧光强度以及设计适配体为G四联体的荧光探针开关具有借鉴意义。 |
| 中文关键词:DNA/银纳米簇 互补配对 荧光探针 适配体 |
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| The effect of different guanine base number on fluorescence intensity of DNA/ silver nanoclusters |
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| Abstract:Objective To investigate the effect of different guanine base numbers on the fluorescence intensity of DNA/ silver nanoclusters through C-G base complementary pairing, in order to explore a new method for the construction of fluorescent probe switches.Methods Designed complementary sequences of aptamer parts of a series of silver nanoclusters by using the nucleic acid base complementary pairing principle, and investigated the effect of the number of G bases exposed in the aptamer sequence on the fluorescence signal.Results Base G could enhance the fluorescence signal intensity of silver nanoclusters, and the fluorescence signal strength was positively correlated with the number of G bases. The fitting linear equation was Y=1726.1X+8972.5, r=0.9789.Conclusion This study is a great reference for the regulation of fluorescence intensity of silver nanoclusters and the design of G quadruplet aptamer fluorescent probe switch. |
| keywords:DNA/AgNCs complementary pairing fluorescence probe aptamer |
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